血管紧张素II1型受体基因多态性与原发性高血压的关系

Association of angiotensin II type 1 receptor gene polymorphism with essential hypertension.

目的探讨血管紧张素Ⅱ1型受体（AT1R）基因多态性与原发性高血压（EH）之间的关系。方法应用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）对200例汉族EH患者（EH组）和192例正常血压者（对照组）的ATlR基因1166A／C及-810A／T多态性进行检测，测定空腹血糖、甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL—C）及高密度脂蛋白胆固醇（HDL—C）等生物化学指标，分析各基因型和等位基因频率与EH的关系。结果1166A／C等位基因和基因型频率在EH组和对照组的分布无统计学差异（P均〉0．05），-810A／T各基因型在EH组和对照组间差异有统计学意义（χ^2=10．862，P=0．004），-810T等位基因频率在EH组显著增高[22．5％（102／400）与11．5％（44／384），χ^2=12．745，P=0．000]，用Logistic回归模型校正了传统危险因素的影响后，-810AT和TT基因型的携带者患高血压的危险性显著增加（P=0．003，OR值为2．57，95％CI：1．37～4．84）。结论AT1R-810A／T多态性与EH发病相关，-810T等位基因可能是EH发病的风险因子。

Objective To investigate whether angiotensin II type 1 receptor（AT1R）gene polymorphism is associated with essential hypertension（EH）. Methods A total of 200 hypertension patients and 192 normotensive controls were enrolled. The AT1R gene 1166A/C and -810A/T polymorphism were determined by polymerase chain reaction-restriction fragment length polymorphism （PCR-PFLP）, and the association between the SNPs and the EH were analyzed statistically. Some biochemical index such as serum glucose （ GLU ） and total cholesterol （ TC ）, triglyceride （TG）, high density lipoprotein （HDL-C） and low density lipoprotein （LDL-C） were also measured. Results There was no significant difference between two groups of 1166A/C polymorphisms of AT1R gene （P 〉 0.05 ）. However, for the -810A/T polymorphism of AT1 R gene, -810 AT and TT genotypes frequencies were significantly higher in EH patients than control （P = 0. 004 ）. The -810T allele frequencies were higher in case than in control （22.5% vs. 11.5% ;P = 0. 000）. We also found an association between EH and -810AT and TT genotypes by logistic regression analysis （ P = 0. 003 ）, adjusted for other risk factors. The odds ratio was 2.57 （95% CI：1. 37 -4. 84）. Conclusions AT1R -810A/T polymorphism is associated with EH and -810T allele may be a risk factor of hypertension