HBV—DNA和HBV—M定量检测的相关性研究

Correlation of quantititive measurements between HBV-DNA and HBV-M.

目的探讨HBV—DNA和乙型肝炎五项（HBV—M）定量检测之间的相关性及其在乙型肝炎感染诊断中的应用价值。方法收集我院363例乙型肝炎和既往感染HBV患者血清，实时荧光定量PCR检测HBV—DNA，同时以时间分辨免疫荧光技术（TRFIA）定量检测HBV-M，用统计软件分析两者之间的关系。结果乙型肝炎大三阳（HBsAg、HBeAg、HBcAb阳性）患者外周血HBV—DNA阳性率为92．9％（79／85），平均DNA含量（4．31±1．64）×10^6Copies／ml。乙型肝炎小三阳患者（HBsAg、HBeAb、HBcAb阳性）外周血HBV．DNA阳性率为47．7％（105／220），平均DNA含量（2．47±2．21）×10^4Copies／ml。HBsAg、HBcAb阳性3例，HBV-DNA均阳性，HBV—DNA为（5．73±1．14）×10。Copies／ml。余人群外周血HBV—DNA均阴性。HBV—DNA拷贝量与HBeAg载量呈正相关（r=0．59，P=0．041）；HBV。DNA拷贝量与HBsAg含量相关一致性不明显（r=0．221，P=0．077）。结论HBV—M与HBV—DNA之间既有联系又有不同，临床上可以多项检测来估计病情，判断疗效。

Objective To explore the correlation between HBV-DNA and serum HBV markers quantitative detection and determine its diagnosis value in hepatitis B virus infection. Methods The HBV-DNA was measured by fluorescence quantitative PCR and hepatitis B virus was detected by time-resolved fluorescence immunoassay （TRFIA）. The relationship between the two tests was analyzed statistically using software. Results Detection of HBV-DNA in the peripheral blood of patients with HBsAg （ ＋ ）, HBeAg （ ＋ ）, HBcAb （ ＋ ） showed 92.9% （79/85） positive rate,and the average DNA copy number was （4.31 ＋ 1.64） × 10^6 copies/ml. While the detection of HBV-DNA in the peripheral blood of patients with HBsAg （ ＋ ）, HBeAb （ ＋ ） , HBcAb （ ＋ ） showed 47.7% （105/220） positive rate, and the average DNA copy number was （2.47 ± 2. 21 ） × 10^4 copies/ml. In normal controls,blood HBV-DNA detection was negative. Furthermore, HBV-DNA copy number correlated positively with HbeAg quantitation （r =0.59,P = 0. 041 ）. However, we found no significant correlation between HBV-DNA copy number and HbsAg quantititation （r = 0. 221 ,P = 0. 077）. Conclusions Different HBV-M had diverse correlation with HBV-DNA number,comprehensive detection of them would be better to assist clinical diagnosis and evaluate the response to treatments.