培养大鼠主动脉血管平滑肌细胞产生巨噬细胞集落刺激因子及其受体的表达

MACROPHAGE COLONYSTIMULATING FACTOR PRODUCTION AND cfms EXPRESSION IN CULTURED RAT AORTIC VASCULAR SMOOTH MUSCLE CELLS*

本研究采用培养大鼠主动脉血管平滑肌细胞（ＶＳＭＣｓ），结果如下：（１）用生物活性检测法发现ＶＳＭＣｓ无血清条件培养液可刺激巨噬细胞集落形成，其作用能被抗巨噬细胞集落刺激因子（ＭＣＳＦ）抗体抑制；（２）用免疫细胞化学技术证明ＶＳＭＣｓ存在ＭＣＳＦ受体；（３）用Ｎｏｒｔｈｅｒｎｂｌｏｔ技术证明ＶＳＭＣｓ有ＭＣＳＦ及其受体的ｍＲＮＡ表达，血清刺激使两者表达明显增强。本研究首次报道了培养大鼠主动脉ＶＳＭＣｓ能合成并分泌具有生物活性的ＭＣＳＦ，而且该细胞存在ＭＣＳＦ受体。上述结果提示，ＭＣＳＦ可能以自分泌或旁分泌方式调节ＶＳＭＣｓ功能，为研究ＭＣＳＦ对于ＶＳＭＣｓ的增殖作用及其机制提供合适的细胞模型。

The resulats of this study are as follows. (1) As measured by a bioassay, a macro￣phage colonystimulating activity was detected in the serumfree conditioned medium of rat aortic vascular smooth muscle cells (VSMCs), which could be subdued by the addition of specific anti macrophage colonystimulating factor (MCSF) antibody. (2) The presence of MCSF receptor was confirmed by immunocytochemistry using a specific anti cFms antibody. (3) The presence of mRNAs for MCSF and cfms (which encoded MCSF receptor) was determined by Northern blot analysis. Their expressions were detectable in quiescent VSMCs and markedly increased after addition of serum. These data dem￣on￣strated for the first time the production of MCSF and the presence of MCSF receptor in cultured rat VSMCs. It is suggested that MCSF might modulate VSMCs functions via both autocrine and paracrine mechanisms. Rat VSMCs appear to be a suitable cell model for studying the cell proliferation effect of MCSF.