强脉冲光改善慢性长波紫外线所致鼠皮肤光老化的研究

Improvement of intense pulse light irradiation on photoaging skin in Balb/c mouse

目的:通过慢性长波紫外线(UVA)照射小鼠皮肤形成光老化损伤,探讨强脉冲光(IPL)改善皮肤光老化的可能作用机制。方法:将实验所用BALB/c小鼠按不同处理条件分为6组:空白组1、UVA组、8-甲氧补骨脂素(8-MOP)+UVA组、UVA+IPL组、8-MOP+UVA+IPL组和空白组2。UVA或8-MOP+UVA连续照射12周,继之IPL照光2次,间隔2周。取小鼠皮肤组织行组织病理检查及透射电镜检查。结果:与空白组比较,UVA组小鼠皮肤真皮层变厚,可见胶原组织变性。8-MOP+UVA组小鼠皮肤表皮层亦见增厚,真皮层厚度的增加和异常胶原纤维的堆积较UVA组更显著,电镜下可见线粒体空泡化变性,胶原纤维溶解。IPL处理后,小鼠皮肤表皮层未表现明显增厚,真皮层扭曲变性的胶原纤维结构得到改善,胶原纤维排列重新趋于整齐紧密,电镜下组织受损明显得到改善。结论:8-MOP可以加速促进光老化进程,慢性UVA照射+8-MOP可成功快速诱导小鼠皮肤的光老化损伤。IPL治疗能够有效改善小鼠皮肤光老化。

Objective： To investigate the improving effects of intense pulse light （IPL） on the skin of photoageing model mice induced by long-term ultraviolet A or 8-MOP＋UVA irradiation. Methods： The BALB/e mice were randomly divided into 6 groups with different experimental intervals： control group 1, UVA group, 8-MOP＋UVA group, UVA＋IPL group, 8-MOP＋UVA ＋IPL group and control group 2. After 8-MOP＋UVA and/or IPL treatment for terminals, the irradiated skin specimens were taken for histopathology ex amination by light microscopy and electron microscopy. Results： Compared with the control group, the epidermis showed a little thick- er and the collagen tissue in the dermis became degenerated in UVA group. These changes became more remarkable in 8-MOP＋UVA group. Under the intervention of IPL, the photoaging changes of skin tissue could be improved obviously. Conclusions： The murine photodamage mouse skin can be established better and effectively by means of 8-MOP＋UVA. IPL has the protective potency on the skin of photoaging mice.