一氧化氮对大鼠背根神经节瘦素长型受体表达的影响

Effect of nitric oxide on the expression of long type of leptin receptor in dorsal root ganglion of rat

目的研究一氧化氮（NO）对大鼠背根神经节（DRG）神经元中瘦素长型受体（OB-Rb）mRNA和蛋白表达的影响。方法用一氧化氮供体硝普钠（SNP;0、0.01、0.1和0.5 mmol/L）处理体外培养大鼠DRG神经元;用RT-PCR和免疫印迹技术检测神经元中瘦素长型受体mRNA和蛋白的表达。结果0.1~0.5 mmol/L的SNP能促进瘦素长型受体mRNA和蛋白表达。结论NO对DRG的功能调节可能与瘦素相关。

Objective To investigate the effect of nitric oxide（NO） on the expression of long type leptin receptor（OB-Rb） in dorsal root ganglion（DRG） neurons.Methods Sodium nitroprusside（SNP;0,0.01,0.1 and 0.5 mmol/L） were used to treat the DRG neurons cultured in vitro.RT-PCR and Western blot were used to detect the expression of long types of leptin receptor（OB-Rb） in DRG neurons.Results 0.1～0.5 mmol/L SNP can increase the expression of OB-Rb mRNA and protein.Conclusion The modulation of NO on DRG neurons may be related with the function of leptin.