摘要
以朵丽蝶兰为材料，对乙烯和生长素调节的授粉后花的发育进行了研究。实验结果显示，切花和植株上的花授粉后，乙烯的产生和花的发育无明显差异；花瓣的衰老、子房发育、花粉萌发和花粉管的伸长受乙烯调节；与切花相比，植株上花的子房内无ＡＣＣ合酶和ＡＣＣ 氧化酶ｍＲＮＡ 的积累。用生长素运输抑制剂２ ［（１ｎａｐｈｔｈａｌｅｎｙｌａｍｉｎｏ）ｃａｒｂｏｎｙｌ］ ｂｅｎｚｏｉｃａｃｉｄ（ＮＰＡ） 处理柱头，授粉诱导的子房发育在很大程度上受到抑制， 表明授粉后子房的发育需要转运来的生长素。
The regulation of postpollination development by auxin and ethylene was examined in Doritaenopsis flowers. These results showed that there were no detectable differences in pollination induced ethylene production and pollination responses regulated by ethylene between detached and attached flowers. The effects of inhibitors of ethylene biosynthesis on early postpollination changes confirmed that perianth senescence, ovary development, pollen germination and pollen tube growth were regulated by ethylene. It has also been found that the flowers attached to the plant did not exhibit ACC synthase and ACC oxidase gene expression in the ovary. Pretreatment of the stigma with 2[(1 naphthalenylamino)carbonyl] benzoic acid(NPA), an auxin transport inhibitor, blocked pollination induced ovary development to a large extent, suggesting that the transport of auxin is required for normal ovary development.
关键词
授粉
乙烯
生长素
发育
花
pollination, ethylene, ACC synthase, ACC oxidase, auxin, development
