用量热法研究水稻线粒体不同条件下的能量释放

Studies on the Energy Liberation of Rice Mitochondria Under Different Conditions by Means of Calorimetry

用精密微热量计（ＬＫＢ２２７７ 生物活性检测系统） 和差示扫描量热（ｄｉｆｆｅｒｅｎｔｉａｌ ｓｃａｎｎｉｎｇ ｃａｌｏｒｉｍｅｔｒｙ， ＤＳＣ） 仪（Ｄｕｐｏｎｔ９１０） 分别测定了广丛４１Ｂ 水稻线粒体体外能量释放热谱和ＤＳＣ 曲线。探讨了它在恒温和变温条件下能量释放的规律和特性，建立了广丛４１Ｂ水稻线粒体在不同条件下能量释放的动力学模型，计算了它在能量释放过程中的热力学和动力学参数，并比较了它们之间的差异。结果表明，温度愈低，线粒体能量释放速率愈慢。线粒体在零上低温下放置较长时间后，其放热量增加，能量释放速率加快。

This paper reported the thermodynamic and kinetic behaviours of the energy liberation of the mitochondria isolated from Guangchong 41 fertile lines of rice (Oryza sative L.) studied by using an LKB 2277 bioactivity monitor and a Dupont 910 differential scanning calorimeter (DSC) & 9900 computer/thermal analyzer system under constant and changing temperature. The thermograms of energy liberation of the rice mitochondria (which had been kept at 0～3℃ for 15 h and 40 d) were determined respectively at 25 and 30℃ (Figs.l,2), and the difference in shape of the thermograms and thermodynamic and kinetic characteristics were compared. The results indicated that the lower the temperature, the slower the energy liberation rate of the rice mitochondria. Both the heat release and the energy liberation rate of the rice mitochondria increased after the mitochondria had been kept at lower temperature for long time (Tables 1,2). In this work, we also determined the DSC curve of the rice mitochondrial energy liberation under changing temperature (Fig.3), and calculated the thermodynamic and thermodynamic and kinetic characteristic parameters of the rice mitochondrial energy liberation under changing temperature (Tables 3,4). One can use these results to characterize the energy liberation ability of the rice mitochondria under different conditions.