摘要
Fresh potato (Solanum tuberosum L.) tuber slices had no capacity of the alternative respiration pathway (ARP). But this capacity could be induced drastically by a 24 h aging process. Western blotting results with a monoclonal antibody against the alternative oxidase (AOX) showed no evidence of AOX in fresh slices. However, an AOX protein of 36 kD was clearly detected in slices aged for 12 or 24 h(Fig.1). Under treatments with ACC and with CoCl 2, the ARP capacity of aged potato slices was enhanced and reduced, respectively. Correspondingly, the extent of AOX protein’s expression was enhanced by ACC and reduced by CoCl 2. The above results give evidence that the regulatory effect of endogenous ethylene on the development of ARP capacity in aging potato tuber slices is exerted through the induction of the expression of AOX therein.
Fresh potato (Solanum tuberosum L.) tuber slices had no capacity of the alternative respiration pathway (ARP). But this capacity could be induced drastically by a 24 h aging process. Western blotting results with a monoclonal antibody against the alternative oxidase (AOX) showed no evidence of AOX in fresh slices. However, an AOX protein of 36 kD was clearly detected in slices aged for 12 or 24 h(Fig.1). Under treatments with ACC and with CoCl 2, the ARP capacity of aged potato slices was enhanced and reduced, respectively. Correspondingly, the extent of AOX protein's expression was enhanced by ACC and reduced by CoCl 2. The above results give evidence that the regulatory effect of endogenous ethylene on the development of ARP capacity in aging potato tuber slices is exerted through the induction of the expression of AOX therein.
基金
国家自然科学基金
关键词
乙烯
交替氧化酶
陈化
马铃薯
切片
endogenous ethylene, alternative oxidase, aging, potato tuber slices
