团花树木葡聚糖转葡糖苷酶cDNA克隆及序列分析

Cloning and sequence analysis of cDNA encoding xyloglucan endotransglycosylase from Anthocephalus chinensis

以团花树形成层组织中大量表达的RNA为材料,通过反转录、保守区域PCR、3′RACE和染色体步移等技术获得了团花树AcXET的全长cDNA序列,共1396bp。核苷酸序列分析表明:该序列含有1个960bp的开放阅读框,编码1个由320个氨基酸组成的蛋白质。该基因编码蛋白与已报道的其他植物的XET蛋白有较高的同源性,且含有XET的活性催化位点EIDFE。

In this paper,we cloned the full cDNA sequence of AcEXT gene,which was copiously expressed in the cambium of Anthocephalus chinensis through reverse transcription,conserved PCR,3＇ RACE and chromosome walking.DNA sequence analysis showed that the 1 396 bp full cDNA sequence included a 960 bp complete open reading frame,which encoded a 320 amino acids protein.The deduced amino acid sequence of AcXET was homologous to the other known XET proteins and contained the conserved EIDFE catalytic site,which was specific to all of the XETs.Results of this paper paved the way for AcXET gene molecular mechanism research during wood formation and cell wall engineering of wood plants in the future.