杂交构树叶片愈伤诱导及植株再生

Callus induction and plantlet regeneration from leaves of hybrid Broussonetia papyrifera

以杂交构树试管苗叶片为外植体,探讨了不同植物生长调节剂组合、叶片着生部位、GA3浓度对杂交构树不定芽再生的影响,建立了叶片不定芽再生体系,为今后的遗传转化奠定了基础。结果表明:杂交构树叶片愈伤诱导和不定芽分化的最适培养基是MS+6-BA2.0mg/L+NAA0.1mg/L,愈伤诱导率为100%,不定芽再生率为91.7%;叶片培养最佳取材部位是自试管苗顶端向下伸展叶第l～3片叶;培养基添加0.5mg/LGA3,芽增殖系数高达8.22,芽有一定的伸长生长;附加低浓度的6-BA0.3mg/L,芽明显伸长,长为3.89cm,得到壮苗,可直接用于生根培养;生根培养添加NAA1.0mg/L,诱导生根的时间最短为9d,生根率最高,达86.7%。

The effects of different factors,such as different combinations of plant growth regulators,position of leaves on seedling and GA3 concentrations,on shoot regeneration from leaves of hybrid Broussonetia papyrifera were studied.The results indicated that the optimum medium for callus induction and shoot regeneration was MS ＋6-BA 2.0 mg/L ＋ NAA 0.1 mg/L,the callus induction rate could reach 100% and the frequency of adventitious bud regeneration was 91.7%.The best leaves for adventitious bud regeneration were the first three top leaves of a shoot.When MS medium was supplemented with 0.5 mg/L GA3,the shoot propagation coefficient reached 8.22 and the length of shoots increased.When low concentration of 6-BA（0.3 mg/L） was supplemented to MS medium,the length of shoots was 3.89 cm,which was significantly increased,and the adventitious bud could be used for rooting culture directly.When NAA（1.0 mg/L） was added in MS medium,the rooting time was only 9 days and rooting rate reached 86.7%.