摘要
以悬浮培养的胡萝卜（ＤａｕｃｕｓｃａｒｏｔａＬ．）与烟草（ＮｉｃｏｔｉａｎａｔａｂａｃｕｍＬ．ｃｖ．ＢＹ２）细胞原生质体为材料，加入一定浓度的细胞色素ｃ和ｄＡＴＰ。不同取样时间的ＤＡＰＩ荧光染色与电镜超薄切片观察的结果显示染色质发生凝集、趋边化，最终形成凋亡小体。核酸电泳显示ＤＮＡ发生特异降解并形成电泳“阶梯”（ＤＮＡｌａｄｄｅｒ）。用末端脱氧核糖核酸转移酶介导的ｄＵＴＰ切口末端标记方法（ＴＵＮＥＬ）检测发现ＤＮＡ的３＇ＯＨ断端被原位特异标记。以上结果说明：细胞色素ｃ能诱导植物细胞发生典型的凋亡。
The study revealed the detection of stereotypic hallmarks of the apoptosis during cell death induced by cytochrome c in carrot (Daucus carota L.) and tobacco (Nicotiana tabacum L.) protoplasts. The progressive delineation of fragmented DNA into distinct bodies, coincident with the appearance of DNA ladders, was also observed during death of the cytochrome c treated carrot and tobacco protoplasts. TUNEL analysis of cells revealed DNA fragmentation localized in the dying cells as well as the additional formation of apoptotic like bodies. These results suggest that cytochrome c can induce programmed cell death (PCD) in plant cells; also indicate that PCD in plants involves some of the characteristics of animal apoptosis among which DNA cleavage is a potential indicator of PCD in plants.
