柑橘与黄皮属间体细胞杂种植株的异常倍性变异

Unexpected Ploidy Variation of the Somatic Hybrid Plants Between Citrus sinensis and Clausena lansium 

黄皮属（Ｃｌａｕｓｅｎａ）是芸香科柑橘亚科黄皮族植物，有３０余个种，其中１１个种原产于中国。黄皮在我国广东、广西、福建等省区有大面积商业栽培，其中鸡心黄皮是最著名的品种［１］。本研究以柑橘属优良脐橙品种“朋娜”为胚性愈伤组织亲本，以黄皮属品种鸡心黄皮...

Wide somatic hybridization was conducted between diploids of embryogenic protoplasts isolated from elite “Bonanza” navel orange ( Citrus sinensis (L.) Osbeck) and leaf protoplasts from edible “Chicken Heart” Chinese wampee ( Clausena lansium (Lour.) Skeels). Fusion products regenerated into shoots after 8 months of culture. Most shoots unexpectedly rooted well. The hybrid shoots were initially unifoliate, then bifoliate and/or trifoliate leaves appeared probably inheriting from the compound leaf character of Cl. lansium. More than ten randomly selected shoot and root tips were not 2n=4x=36 as expected, but 2n=6x=54 as identified by chromosome counting, suggesting that chromosome doubling occurred rather than chromosome elimination in this remote fusion combination. The hybridity of embryoids, unrooted and rooted shoots was revealed by RAPD analysis. This is the first report on hexaploid somatic hybrid plants regenerated from fusion between diploids in Aurantioideae. The possible reason for the unexpected ploidy variation was also discussed.