高脂血症大鼠肝肾阴虚证动物模型实验研究

Experimental Study on Liver-Kidney Yin Deficiency Syndrome Model in Rat with Hyperlipidemia

[目的]建立一种可操作性、重复性好的高脂血症大鼠肝肾阴虚证动物模型。[方法]将24只SD大鼠分为模型组和正常组,模型组12只予以高脂饮食及长期激怒,模拟高脂血症及中医肝肾阴虚证的病因,复制高脂血症大鼠肝肾阴虚证动物模型。[结果]模型组大鼠出现明显消瘦、活动减少、竖毛少泽、食欲减少、弓背、扎堆等现象,生化检测结果TC(总胆固醇)、TG(甘油三酯)、LDL-C(低密度脂蛋白-胆固醇)显著升高;血浆cAMP(环腺苷酸)含量升高,E2(雌二醇)、E2/T比值显著升高。[结论]高脂饮食配合长期激怒,可使大鼠的症状、实验室指标均能体现所模拟的高脂血症肝肾阴虚证模型,符合临床实际,可将该方法作为中医高脂血症肝肾阴虚证动物模型的制作方案。

[Objective]To establish a feasible and reproducible liver-kidney yin deficiency syndrome animal model in hyperlipidemic rat.[Methods]24 SD rats were randomly divided into model group and control group.12 rats in the model group were fed with high fat diet and long-term irritated,mimicking the etiology of hyperlipidemia and TCM liver-kidney yin deficiency syndrome,to generate a liver-kidney yin deficiency syndrome model of hyperlipidemia.[Results]Rats of model group were with obvious emaciation,less activity,piloerection,less smooth,decreased appetite,back arched,and gathering.Biochemical test showed that TC,TG and LDL-C were significantly higher.cAMP content in plasma was significantly higher.The ratio of E2 and E2/T were significantly higher.[Conclusions]High fat diet combined with long-term irritation can reflect the mimic rat liver-kidney yin deficiency model of hyperlipidemia in terms of symptoms and laboratory parameters.This method conforms to clinical practice and can be used as a TCM liver-kidney yin deficiency model of hyperlipidemia.