金属硫蛋白导向血栓单抗化学偶联体的制备及性质研究

Preparation and Characteristics of Metallothionein and Thrombus directed Antibody Chemical Conjugates

金属硫蛋白（ｍｅｔａｌｏｔｈｉｏｎｅｉｎ，简称ＭＴ）是一类低分子量、富含半胱氨酸的蛋白质，由于１个ＭＴ分子可结合７个金属离子，因此ＭＴ分子具有很强的金属结合特性［１，２］．本文一方面利用ＭＴ分子的高金属结合性能，另一方面利用苏州医学院血液研究所研制成...

In order to make the detection of thrombus faster and more sensitive,two chemical conjugates of metallothionein and SZ51 which is an anti human activated platelet in thrombus were obtained through protein coupling method.In the crosslinking process,two coupling reagents were used,one being glutaraldehyde,and the other ethylcarbodiimidhydrochlorid.The results showed that conjugate crosslinked by glutaraldehyde kept lower antibody activity,while the antibody activity of conjugate crosslinked by ethylcarbodiimidhydrochlorid was the same as the original antibody SZ51;the two conjugates had higher metal ions binding ability.Both of the conjugates provided directing and imaging reagents for detection of thrombus.