VCAM-1在急性心肌缺血再灌注损伤中表达的意义

Significance of VCAM-1expression in acute myocardial ischemia and reperfusion injury

目的:观察急性心肌缺血再灌注后VCAM-1在心肌组织中表达的规律,探讨急性心性猝死的诊断指标。方法:用免疫组织化学方法观察兔心肌缺血再灌注后VCAM-1蛋白在不同实验组中的表达规律,以正常组作为对照。结果:①正常对照组:VCAM-1蛋白在心肌组织中有低水平表达;②心肌缺血组:心肌组织中均可观察到VCAM-1蛋白的表达;③心肌缺血再灌注组:心肌缺血再灌注0.5 h,即可发现VCAM-1蛋白的表达;心肌缺血再灌注1 h,心肌组织中VCAM-1的表达增强,心肌缺血再灌注2 h,心肌组织中VCAM-1蛋白的表达至高峰;心肌缺血再灌注4 h,心肌组织中VCAM-1蛋白的表达均逐渐下降。结论:在心肌缺血再灌注时,心肌组织中有VCAM-1的表达,并随时间呈一定规律性变化。

Objective：To establish the diagnostic criteria for sudden cardiac death by examining the regular pattern of VCAM-1expression in acute ischemia-reperfusion myocardium.Methods：The experimental rabbits were divided into groups of normal controls,ischemia or ischemia-reperfusion（I/R）,and immunohistochemical technique was used to determine the expression of VCAM-1.Results：VCAM-1 expression in myocardium was found in both groups of the controls and ischemia,but it was weakly positive in the normal controls.In I/R group,the positive expression of VCAM-1 was observed at 0.5h after reperfusion,but increased at 1h,with peak at 2h,and yet,the expression declined at 4h after the intervention.Conclusion：VCAM-1 expression may be found during acute ischemia-reperfusion injury,showing itself by regular pattern with time.