子宫内膜异位症的免疫状态与中医辨证分型的关系

Relation between Immune Function of Endometriosis and Traditional Chinese Medicine Syndrome

目的探讨子宫内膜异位症（ＥｎｄｏｍｅｔｒｉｏｓｉｓＥＭＳ）的免疫状态与中医辨证分型的关系。方法采用间接免疫荧光法检测ＥＭＳ患者外周血中的Ｔ细胞亚群、Ｂ、ＮＫ、ＩＬ－２Ｒ＋细胞，并用酶联免疫双抗体夹心法检测外周血及腹腔液中的ＴＮＦ－α，并与中医辨证分型进行相关关系分析。结果以气滞血瘀、寒凝血瘀、湿热瘀阻三组组成的实证组ＣＤ＋４／ＣＤ＋８值升高，而肾虚血瘀组除此变化外，Ｂ、ＩＬ－２Ｒ＋细胞百分率增高，外周血及腹腔液ＴＮＦ－α浓度也显著增高。结论ＣＤ＋４／ＣＤ＋８值与多因素逐步回归分析后发现，ＥＭＳ患者外周血中ＣＤ＋４／ＣＤ＋８值与中医辨证分型密切相关。

Objective :To study the relation between immune function of endometriosis (EMS) and traditional Chinese medicine syndrome. Method:Peripheral blood leukocyte profile of endometriosis were observed by using indirect immunofluorescence assay, ELISA was further applied to determine the conceration of TNF a in the parallel samples of peripheral blood and peritoneal fluid. Results: After distinguishing the syndromes of the EMS patients according to traditional Chinese medicine theory, it is discovered that in the sthenia syndrome group, the ratio of CD 4 +/CD 8 + is higher than that of control group, these variations in the kindeny deficiency blood stasis group, the variation of the ratio of CD + 4/CD + 8 is ,more significant than that of the sthenia syndrome group, Besides, the percentage of B cells and IL-2R + cell and concentration of TNF α are highr than that of the control group. Conclusion:Multifactors logistic regression to the ratio of CD + 4/CD + 8 shows that the ratio of CD + 4/CD + 8 is closely relation with the syndromes of traditional Chinese medicine.