辣椒素治疗截瘫后下肢肌紧张度增高的实验研究

Experimental Research of Therapeutic Effect of the Capsaicin on the Hypermyotension in the Paralyzed Limbs

本实验的目的在于用皮下注射辣椒素的方法耗竭脊髓后角Ｐ物质，以观察其对下肢肌肉紧张度增高的治疗作用。实验用Ｗｉｓｔａｒ大鼠，在Ｔ５水平做脊髓横断术以建立大鼠模型，观察脊髓横断前后股四头肌自发性放电的变化情况，并以皮下注射辣椒素２００ｍｇ／ｋｇ体重。观察５天后肌电、痛阈及一般状态的变化，最后用放免法测定脊髓后角Ｐ物质含量。结果表明，用辣椒素处理动物后，脊髓后角Ｐ物质含量下降３８．４％，实验组肌电明显低于对照组，痛阈增高，对一般状况无显著影响。说明皮下注射辣椒素耗竭脊髓后角Ｐ物质能够有效地缓解下肢肌肉紧张度的增高。

In order to observe the changes of myotension in the paralyzed limbs after subcutaneous injection(S.C)of capsaicin,we observed the changes of the spontaneous Electromyography(EMG)recorded in the quadriceps femoris before and after T 5 level spinal transection and found that the amplitude of EMG kept a low level in normal rats but increased progressively after operation.After treating the animals with capsaicin 200mg/kg(S.C)in three consecutive days,we found a much lower spontaneous EMG and a much higher pain threshold than that of the controls.Radioimmunnoassay detected that the content of SP in spinal dorsal horn was reduced by 38.4%,the general condition did not severely changed during the experiment.The results indicate that SP in the spinal dorsal horn participates in the regulation of myotension,and it is suggested that subcutancous injection of capsaicin may be one effective treatment to reduce the hypermyotension in the paralyzed limbs.