HPLC测定小儿喜食糖浆中柚皮苷含量

HPLC Determination of Naringin in Citrus Fruit for Children Happily to Eat

建立了HPLC法测定小儿喜食浆中柚皮苷含量测定方法.采用18烷基硅烷键合硅胶为填充剂,流动相:乙腈-水(21.5:80.5),流速1.0ml/min,检测波长283nm.柚皮苷的线性范围为50-800μg/ml,平均回收率98.8%,精密度相对标准偏差(RSD)为0.41%,重现性RSD为1.1%,稳定性RSD值为0.32%.本方法简便、准确,可供本品质量控制.

Objective：To establish the quantitative method of naringin in citrus fruit for children happily to eat by using HPLC.Method：To adopt ODS-C18（150mm × 4.6mm,5μm）.Mobile phase,Acetonitrile-water（21.5：80.5） make ph：3.0 By phoric acid,flow rate at 1.0ml/min,wavelength 283nm,Result：The linear range 50-800μg/ml,the average recovery 98.8%,precision RSD 0.41%,repetition RSD 1.1%,stability RSD 0.32% 。 Conclusion：This method is accurate and reliable