摘要
目的与方法：本实验研究旨在通过对菌丝体直径的测量和分析天平称重的方法比较，寻找高产深层富硒灵芝菌丝体的最佳培养方法；结果：用葡萄糖作碳源，在５００ｍＬ的三角烧瓶中装入２００ｍＬ的培养基、接种母液含量为１５％，封口材料用６层纱布，温度２８℃，摇床转速２００ｒｐｍ，亚硒酸钠浓度０．０１ｇ％；结论：使用此培养条件培养的富硒灵芝，不仅产量高，而且经采用微量元素及氨基酸含量分析证明。
Objective
and method: The experiment is intended to explore a way to productively yield the Se
rich Glossy Ganoderma by means of measuring the diameter of the hypha body and
weighing the hypha with an analytical balance on different productive conditions.Result
:The best way revealed by the experiments is that dextrose as carbon source,200ml
culture media in a 500ml flask,15% density of mother liquor,sealed with 6 layers of
gauze,rotated on a table at 200 rpm,0.01% consistency of Na 2Se 2O
3.Conclusion:Under the conditions ,the output is not only high but it is testified by the
microchemical analysis and volume analysis that the medical and nutritive amino acids
of the Se rich Glossy Gandrema is rich.
出处
《河北医学》
CAS
1999年第6期1-3,共3页
Hebei Medicine
关键词
富硒灵芝
有机硒
氨基酸
菌丝体
生长
Se rich Glossy Ganoderma
Organic
Selenium
Amino acids
