针刺对脑缺血再灌注损伤大鼠脑水肿和脑梗死面积变化的调节

Effect of Electroacupuncture on Expression of Cerebral Edema and Cerebral Infarction

目的:观察针刺对脑缺血再灌注损伤大鼠神经功能变化,脑组织含水量、梗死面积的影响。方法:线栓法闭塞SD大鼠大脑中动脉,制作局灶性脑缺血再灌注模型。分为假手术组、模型组、电针组、5-羟葵酸+针刺组(5-HD10mg/kg静脉注射,30min后再行MCAO,n=8)。结果:与假手术组比较,模型组神经功能分值升高,脑梗死面积增大,脑含水量升高,差异显著(P<0.01);与模型组比较,针刺组大鼠的神经行为学评分降低,脑含水量减少,脑梗死面积减少,差异显著(P<0.01);5-羟葵酸+针刺组与模型组基本相似(P>0.05),各指标比较差异无显著性。结论:针刺对脑缺血再灌注损伤大鼠有明显的保护作用,其机制可能与针刺能降低缺血再灌注损伤大鼠脑水肿有关。

Objective：To investigate the influence of electroacupuncture on NIHSS of pallium and the affection of the cerebral water content,the brain index,the cerebral area of infarction.Methods：The I/R models of rats were performed by thread embolism of middle cerebral artery for 2 and 24h of reperfusion.30 male Wister rats were randomly divided into four groups：sham-operated group,ischemia group,the treatment group and Zhen＋5-HD group.The MCAO lasted for 2h and animals were reperfused for 24h.Neuropsychological score,infarct volume by total brain slices calculation and mitochondrial ultrastructure were measured.Result：Comparing with the sham operation group,the Neuropsychological score,infarct volume of the brain,and the brain index of the ischemia group increase.By the same time,mitochondria of the ischemia group are swollen,thickness,and vacuolization.The inside spinal column of the mitochondria break and the film break too.（P〈0.01）;comparing with the ischemia group,the Neuropsychological score,infarct volume of the brain,and the brain index of the Zhen group decrease.Mitochondria of the Zhen group are less swollen,the basic configuration is prefect,and the gap of the inner film is very clear.（P〈0.01）;the value of the Zhen＋5-HD group and the ischemia group are similar the same.Conclusion：The acupuncture significantly effect to prevent the NIHSS of pallium,the mechanism might be the acupuncture would decrease the cerebral water content,the brain index,the cerebral area of infarction.