血浆尾加压素Ⅱ水平与CCL4大鼠肝纤维化程度的关系

Correlation between plasma levels of urotensin II and severity of liver fibrosis in CCi4 rats

目的研究血浆尾加压素Ⅱ（UⅡ）水平与肝纤维化病变程度的关系。方法Wistar大鼠腹腔注射CCL．制备肝纤维化模型。肝纤维化组动物分别在4、6、8周处死。取肝组织测羟脯氨酸含量并观察肝脏病理学改变，采用ELISA方法分析检测UⅡ水平。结果随模型制备时间延长血浆UⅡ水平逐渐升高，并与肝脏纤维化程度呈正相关。结论UⅡ可能是参与肝纤维化发生、发展的重要因素。

Objective To examine the correlation between urotensin Ⅱ（UⅡ） concentration and the se- verity of liver fibrosis. Methods Liver fibrosis model was induced in rats by intraperitoneal administration of CC14. At 4,6,8 weeks, the rats were sacrificed, and the hepatic tissue hydroxyproline （HYP） content was measured. Hepatic tissue specimens were histopathologically evaluated according to a fibrosis scoring sys- tem. Plasma UⅡ levels were measured by ELISA method. Results Plasma UⅡ gradually increased with the increase of duration of CCL4 , UⅡ eoneentration eorrelated to liver fibrosis （R^2 = 0. 875, P 〈 0.05 ） and hepatic HYP（R^2 =0.65, P 〈 0.05）. Conclusion UⅡ was involved in the pathogenesis of liver fibrosis.