摘要
目的探讨CD4+和CD8+T淋巴细胞上PD-1的表达与慢性乙型肝炎患者的关系。方法采用流式细胞技术测定66例慢性乙型肝炎患者、17例HBV携带者外周血CD4+、CD8+T淋巴细胞PD-1,并与19例健康人群对照。结果慢性乙型肝炎患者外周血CD4+T淋巴细胞PD-1的荧光强度较对照组明显增强,而慢性乙型肝炎患者CD8+T淋巴细胞和HBV携带者CD4+T淋巴细胞PD-1的表达量和荧光强度都高于对照组。CD4+和CD8+T淋巴细胞PD-1表达与血清中乙肝病毒载量无明显关系。慢性乙型肝炎轻度组患者CD4+和CD8+T淋巴细胞PD-1表达的荧光强度明显高于重度组,差异有统计学意义(P<0.05)。结论慢性乙型肝炎患者存在细胞免疫功能紊乱和淋巴细胞异常激活现象,CD4+和CD8+T淋巴细胞PD-1的表达与其病情发展和转归相关。
Objective To investigate the programmed death 1 （PD-1）expression of T-lymphocyte in hepatitis B and its clinical significance. Methods The change of PD-1 expression of T-lymphocyte was detected by immunofluoreseent flow cytometry in 66 cases of chronic hepatitis B, 17 HBV carriers and 19 normal control subjects,and serum HBV-DNA viral load was detected by PCR. Results No significant difference in the PD-1 expression level of CD4＋T lymphocyte was observed in patients with chronic hepatitis B and in the normal controls,but the fluorescence intensity of CD4＋ T lymphocyte in HBV carriers was significantly higher than that in controls. The PD-1 expression level and the fluorescence intensity of CD8＋ T-lymphocyte in patients with hepatitis B and HBV carries were significantly higher than those in controls. There was no correlation between PD-1 expression level and HBV viral load. There was no significant difference in the PD-1 expression level of CD4 ＋ and CD8＋ T-lymphocyte in various of condition hepatitis B. But the fluorescence intensity of CD4＋ and CD8＋ T-lymphocyte in mild chronic hepatitis B was significantly higher than that in severe chronic hepatitis B. Conclusion The findings support that cellular immunodeficiency exists in hepatitis B and that T lymphocytes are abnormally activated in chronic hepatitis B,The PD-1 expression level of CD4＋ and CD8＋ T-lymphocyte exerts evident influence on the function of activity and prognosis of chronic hepatitis B.
出处
《中国现代医生》
2010年第29期19-21,共3页
China Modern Doctor
