摘要
应用巢式-PCR方法,对深圳检出的2份HIV-1感染者的外周血单核细胞样本进行扩增,获得HIV-1膜蛋白(env)基因核酸片段,并对其C_2-V_3区及邻区350~450个核苷酸序列进行测定和分析.结果表明这2份血样为HIV-1B亚型毒株感染(sz-B),彼此间的基因离散率为10.2%;与A-E国际参考亚型序列比较,sz-B与A及C-E参考亚型共享序列间的基因离散率均大于22%,而与欧美B亚型(Boon)间的基因离散率仅为7.5%;与国内部分地区流行的B亚型代表株比较其基因离散率为11%~12%.提示HIV-1B亚型在深圳的流行时间约为10年,主要经性途径由境外传入,且经性传播途径在人群中流行.
HIV-1 provirual DNA from peripheral blood mononuclear cells (PBMCs) collected from 2 HIV-1 infected individuals found in Shenzhen was amplified using a nested-PCR. The C2-V3 and flanking region(350-450) nucleotides of HIV-1 env gene were sequenced directly from the PCR products and analysed. These 2 samples were confirmed to be infected with HIV - 1 subtype B strain (sz - B). The innergroup distance of sz -B was 10.2%. In comparison with the consensus sequence of international A -E subgroups and some subtype B epidemic in China, the genetic distances between sz -B and each of A, C - E subgroups were more than 22.0%, 7.5% with subtype B found in America and Europe and 11%-12% with some subtype B epidemic in China. Phylogenetic trees analysis also showed that sz-B clustered into Bcon. These data indicated that the time of sz-B epidemic in Shenzhen was about 10 years, sz -B was mainly transmitted into Shenzhen from abroad and spreaded via sexual transmission route among populations.
出处
《中国性病艾滋病防治》
1999年第3期123-125,共3页
Chinese Journal of Std & Aids Prevention and Control