HIV—1感染者确认实验带型分析

Antibody Detection of HIV by Western Blot

对169份初筛为HIV阳性的血清进行确认实验,发现166份为HIV—1阳性,2份可疑,1份阴性.阳性反应中,外膜蛋白抗原gp160出现频率最高,为100%;多聚酶抗原p66为97%,核心抗原p24为95.2%,提示这三种抗原为HIV-1感染的重要的标志性抗原.其中有20份样本出现HIV—2型反应条带,感染类型需进一步证实.

169 sero samples were tested for the presence of antibodies to HIV by WB. 166 samples were confirmed to be HIV-1 positive, 2 indeterminate, 1 negative. Among the positive reaction, band patterns showed that gp160 was the most frequently detected antigen (166/166, 100%), followed by pol antigen p66 (161/166, 97%) and core antigen p24 (158/166, 95.2%), which indicated that these antigens were the most important labelling antigen of HIV- 1 infection. There were also 20 positive samples reacted with HIV -2 specific antigens, which need to be further identified.