MG132、顺铂联合用药对Caov-3细胞中NF-κB、VEGF表达的影响

Expressions of NF-κB and VEGF in Caov-3 cells after the administration of MG132 combined with DDP

目的研究蛋白酶体抑制剂MG132与DDP联合用药前后卵巢癌细胞株Caov-3细胞内NF-κB、VEGF表达情况。方法免疫组化SP法测定用药前后Caov-3细胞中NF-κB、VEGF的表达情况。结果免疫组化结果显示MG132、DDP联合用药作用24 h后Caov-3细胞中NF-κB、VEGF表达均下调,与对照组、DDP组比较差异有显著性(P<0.01),联合用药组各组间两两比较差异有显著性(P<0.01)。结论 MG132能通过抑制NF-κB活性诱导卵巢癌细胞Caov-3凋亡,抑制肿瘤血管生长。

Objective To study the expressions of NF-κB and VEGF in human ovarian cancer Caov-3 cells after using proteasome inhibitor MG132 combined with DDP.Methods Immunohistochemical assessment was performed for the changes in expressions of NF-κB and VEGF.Results The expression levels of NF-κB and VEGF decreased in Caov-3 cells after being treated with MG132 combined with DDP for 24 hours.The differences were statistically significant（P0.01）.Conclusions MG132 could significantly induce the apoptosis of Caov-3 cells.by inhibiting the activation of NF-κB,and thus inhibite the growth of tumor vessel.