白细胞介素-6、雌激素与绝经后骨质疏松关系的研究

Relationship among Interleukin-6,Estrogen and Postmenopausal Osteoporosis

为探讨妇女白细胞介素－６（ＩＬ－６）及雌激素水平的变化对骨丢失的影响，本文采用四甲基偶氮唑盐微量酶反应比色法（ＭＴＴ）检测青年妇女、围绝经妇女及绝经后妇女的血清ＩＬ－６活性，用放免法检测血清雌二醇（Ｅ２）水平，双能Ｘ线吸收仪测定Ｌ２～４的骨密度（ＢＭＤ）。结果表明：绝经后妇女的血清ＩＬ－６活性明显升高，但血清Ｅ２水平却显著低于青年妇女与围绝经妇女（Ｐ＜０．０１），ＢＭＤ也显著低于青年妇女与围绝经妇女（Ｐ＜０．０１）。相关分析显示，ＩＬ－６与ＢＭＤ呈负相关（ｒ＝－０．７２４，Ｐ＜０．０５），与Ｅ２呈负相关（ｒ＝－０．７５９，Ｐ＜０．０１），Ｅ２与ＢＭＤ呈正相关（ｒ＝０．６６０，Ｐ＜０．０１）。提示，Ｅ２缺乏及其所致的ＩＬ－６水平升高在绝经后骨质疏松的发病机制中起重要作用。

In order to explore the effects of serum interleukin-6 (IL-6) and serum estrogen level on women bone loss, we examined the serum IL-6 activity with MTT assay,serum E2 level with radioimmunoassay and bone mineral density (BMD) at L2-4 using dual-energy X-ray absorptiometer (DEXA) in young women,periclimateric women and postmenopausal women.The results showed that the IL-6 activity was significantly higher in postmenopausal women,whereas the serum E2 level was significantly lower in postmenopausal women compared with young women and periclimateric women(P<0.01);the BMD was also significantly lower in postmenopausal women(P<0.01).Moreever,the IL-6 had negative correlation with BMD (r=-0.724,P<0.05),and negative correlation with E2(r=-0.759,P<0.01);while E2 had positive correlation with BMD(r=0.660,P<0.01).These results suggest that the decrease of E2 and the increased production of IL-6 may play an important role in the pathogenesis of postmenopausal osteoporosis.