滴金免疫测定法(DIGFA)检测血吸虫抗体的研究

THE STUDY ON DETECTING ANTIBODIES OF SCHISTOSOMA BY DOT IMMUNOGOLD FILTRATION ASSAY

目的建立一种简便、快速、敏感、特异的检测血吸虫抗体的新方法。方法用滴金免疫测定法（DIGFA）检测血吸虫病人血清抗体，并以ELISA作平行对照。结果DIGFA和ELISA的敏感性分别为97．1％和95．7％；特异性分别为99．1％和100％，两法无显著差异（P＞0．05）。62份其它寄生虫感染血清，除肺吸虫1例，ELISA呈阳性反应外，其余均为阴性。结论实验结果不仅显示了DIGFA的敏感性和特异性与ELISA相似，而且具有简便、快速、不需要特殊设备等优点，是一种值得推广应用的检测血吸虫抗体的新方法。

ObJective To establish one of simple,fast, sensitive and specific assays for detecting schistosome antibodies. Methods The serum antibodies of patients with schistosomiasis have been detected by dot immunogold filtration assay (DIGFA), and also by ELISA as the parallel coptrol. Results The sensitivities of DIGFA and ELISA are 97.1 % and 95. 7% respectively, and the specificities are 99. 1 % and 100% respectively. There are no significant differences between the two assays (P>0.05). Sixty-two specimens of patients infected with other parasitic diseases are detected by the two assays. All specimens show negetive, except one specimen of a patient infected with Paragonimus westermani detected by ELISA. Conclusion The results suggest that DIGFA is sensitive and specific as the assay of ELISA, and also has other advantages such as simple, fast and without specific equipment. The assay is deserved to apply abroadly in detecting the antibodies of schistosome.