实验性兔动脉粥样硬化发病过程中内源性一氧化碳及其合成酶基因表达的改变

The Research on Endogenous Carbon Monoxide and Expression of Carbon Monoxide Synthase during Atherosclerosis

为研究内源性一氧化碳及其合成酶血红素氧化酶１在动脉粥样硬化发病中的变化规律，通过逆转录－聚合酶链反应、原位杂交、免疫组织化学染色和生物化学等多项指标观察血红素氧化酶１和内源性一氧化碳在动脉粥样硬化发病过程中的变化。结果发现，血红素氧化酶１ｍＲＮＡ主要分布于主动脉和冠状动脉内皮细胞，在动脉粥样硬化的发展过程中其表达有逐渐增高的趋势，且血红素氧化酶１蛋白的表达也有逐渐增高的趋势。血红素氧化酶１免疫组织化学染色显示，主动脉和冠状动脉内皮细胞呈阳性，主动脉壁血红素氧化酶１ｍＲＮＡ的表达量及血红素氧化酶活性和内源性一氧化碳的浓度有逐渐增高的趋势。结果提示，内源性一氧化碳及其合成酶在动脉粥样硬化的发病过程中可能具有重要的作用。

Aim To research the regularity of endogenous carbon monoxide and expression of carbon monoxide synthase (heme oxygenase 1) during atherosclerosis development in rabbits. Methods Reverse transcription-polymerase chain reaction(RT PCR), in situ hybridization, immunohistochemistry stain, and some biochemical index marks were performed so as to research the regularity of endogenous carbon monoxide and expression of carbon monoxide synthase (heme oxygenase 1) during atherosclerosis development. Results The positive score of heme oxygenase 1 mRNA is distributed in endothelial cell of aorta and coronary artery,the express of heme oxygenase 1 mRNA on aorta and coronary artery increase in process of time during atherosclerosis development.the positive score of heme oxygenase 1 protein is distributed in endothelial cell of aorta and coronary artery,the express of heme oxygenase 1 portein on aorta and coronary artery raise in process of time during atherosclerosis development.Through RT PCR method , the express of heme oxygenase 1 mRNA on aorta raise in process of time during atherosclerosis development was observed.The activity of heme oxygenase and the concentration of carbon monoxide increase in process of time during atherosclerosis development. Conclusion Endogenous carbon monoxide and carbon monoxide synthase may play an important role in atherosclerosis development.