月桂氮酮和薄荷醇对复方积雪草苷凝胶体外透皮吸收的影响

Effect of azone and menthol on the in vitro percutaneous permeability of the compound asiaticoside gel

目的:研究不同浓度的月桂氮酮、薄荷醇对复方积雪草苷凝胶体外透皮吸收的影响,以筛选有效的透皮吸收促进剂。方法:采用改进Franz扩散池装置,以离体小鼠鼠皮为透皮屏障,用高效液相色谱法测定接收池中积雪草苷的含量,计算积雪草苷累积透皮吸收量与透皮速率。结果:使用渗透促进剂之后,积雪草苷的渗透速率有明显的提高,其中薄荷醇1.0%>0.5%>2.0%,月桂氮酮1.0%>4.0%>2.0%,月桂氮酮>2种促进剂联用>薄荷醇。以月桂氮酮1.0%的透皮效果最好。结论:本凝胶中含1.0%月桂氮酮时积雪草苷能获得较好的渗透速率。

OBJECTIVE To study the effect of the different concentration of azone and menthol on the percutaneous permeability of the compound Asiaticoside gel in vitro. To screen the effective transdermal absorption enhancer. METHODS Isolated rat skin was taken as the permeable barrier by using a modified Franz absorbing - cell device. The content of Asiatieoside in the modified Franz absorbing-cell device was dertermined by HPLC ,and the cumulation of Asiaticoside permeated through the rat skin was calculated. RGSULTS After adding the penetration enhancer , the penetrating rate of Asiaticoside Gel preparations increased remarkably. Menthol（ 1.0 %〉 0. 5 % 〉 2. 0 % ）, azone （ 1.0%〉 4. 0%〉4%〉 2.0% ）, azone〉 two kinds of penetration enhancers used in combination）menthol. 1.0% azone transdermal was the best. CONCLUSION Asiaticoside gel able to obtain a higher penetration rate when the gel containing 1.0% azone.