上淀粉浆纯棉织物中果胶含量的测定方法

Determination of pectin content on cotton fabric sized with starch

由于果胶对棉织物润湿性影响很大,在前处理过程中,为定量检测处理效果,通常要测定处理后织物上的果胶含量。对于上淀粉浆的纯棉织物,传统的果胶含量测定方法受淀粉浆的干扰严重。在分光光度计DNS显色法的基础上,探索一种新的淀粉内标DNS显色分光光度计测试方法。系统研究了吸收波长、DNS试剂用量、淀粉液硫酸加热回流时间、果胶液硫酸加热回流时间的影响。该方法能够排除淀粉浆的干扰,准确地测定棉织物中果胶的含量,对研究纯棉织物复合酶生物前处理技术以及果胶对棉织物润湿性的影响具有实际意义。

The wetting property of pretreated cotton fabrics is obviously affected by the pectin on cotton fiber.To measure the property of the pretreated cotton fabric in quantity,the content of pectin is usually determined.For cotton fabric sized with starch,the starch has significant impact on the determination of pectin with the traditional test method.In this paper,a new method was proposed on the basis of DNS-spectrophotometer-method.The effects of absorption wavelength,quantity of DNS,reflux time of starch solution with sulfuric acid,and reflux time of pectin solution with sulfuric acid were investigated.The content of pectin can be determined precisely by this method,avoiding the influence of starch.It has practical significance for investigating the pretreatment with compound enzyme and the effect of pectin on wetting property of cotton fabric.