摘要
According to the published nucleotide sequences,CP gene of barley yellow dwarf virus PAV(BYDV-PAV) was synthesized by reverse transcription polymerase chain reaction(RT-PCR).The reporter gene GFP was put to the downstream of whole CP gene sequence of BYDV-PAV after double enzyme digestion,PCR identification and sequencing.The green fluorescence protein expression system of coat protein of BYDV-PAV was successfully constructed and expressed in Escherichia coli BL21(DE3)plysS.The expression product was confirmed by SDS-PAGE,Western blot analysis and fluorescence microscope.The fusion gene CP-GFP was expressed in the range of 15-22℃,but could not be expressed at 23-37℃.The results provided a base for studying on BYDV-PAV movement in aphid further.
According to the published nucleotide sequences,CP gene of barley yellow dwarf virus PAV（BYDV-PAV） was synthesized by reverse transcription polymerase chain reaction（RT-PCR）.The reporter gene GFP was put to the downstream of whole CP gene sequence of BYDV-PAV after double enzyme digestion,PCR identification and sequencing.The green fluorescence protein expression system of coat protein of BYDV-PAV was successfully constructed and expressed in Escherichia coli BL21（DE3）plysS.The expression product was confirmed by SDS-PAGE,Western blot analysis and fluorescence microscope.The fusion gene CP-GFP was expressed in the range of 15-22℃,but could not be expressed at 23-37℃.The results provided a base for studying on BYDV-PAV movement in aphid further.
出处
《植物病理学报》
CAS
CSCD
北大核心
2010年第5期548-551,共4页
Acta Phytopathologica Sinica
基金
公益性行业科研专项(nyhyzx07-051)
转基因抗病毒小麦新品种培育(2009ZX08002-003B)
高等学校学科创新引智计划资助项目(B07049)
