HPLC-共振瑞利散射联用测定庆大霉素各组分

Development of a Novel HPLC-RRS Method for Determination of Gentamicin Components

建立无需衍生的高效液相色谱-共振瑞利散射联用测定庆大霉素（gentamicin,Gen）C组分的方法.滂胺天蓝作为分子识别探针,与Gen生成离子缔合物,产生增强的散射信号,荧光检测器设定λex=λem=356 nm进行检测.实验结果表明：Gen中C1、C1a、C2和C2a 4个主要组分的浓度分别在7.2～115.2、8.2～131.6、7.5～78.8、8.5～78.0 mg/L具有良好线性关系,检出限在6.0～7.2 mg/L.将方法应用于临床使用的Gen注射液测定,结果符合〈中国药典〉2005版规定的范围.

A selective and rapid high-performance liquid chromatography coupled with resonance Rayleigh scattering detection method was developed for the determination of multicomponent gentamicin （Gen）. Pontamine sky blue was chosen as molecular recognition probe,which could forms ion-association complex with Gen in mobile medium and produce strong scattering signal. The separation of Gen components was achieved on a Cls column and the column eluent was monitored using fluorescence detection at λem=λm=356 nm. The detection limits of 6.0-7.2 mg/L were reached,and the response linearity between peak area and components concentration were found in the range of 7.2 - 115,8. 2- 132,7.5- 79 and 8.5 - 78 mg/L for C1,C1a,C2,C2a,respectively. This method has been applied to the determination of Gen components in commercial Gen sulfate injection,and the detection results were in good agreement with the specification of Chinese Pharmacopoeia.