摘要
目的：研究不同透析膜和内毒素对尿毒症患者外周血单个核细胞白细胞介素１β（ＩＬ１β）基因表达和蛋白质合成的影响。方法：细胞和不同透析膜孵育后采用原位杂交检测ｍＲＮＡ水平，单个核细胞培养后酶联免疫吸附试验法测定细胞总蛋白质水平。结果：不同透析膜孵育后的单个核细胞在未用内毒素刺激情况下ＩＬ１βｍＲＮＡ水平有显著差别，但蛋白质合成量无差异，其中正常人仅有极微量ＩＬ１β合成；内毒素刺激下的单个核细胞ＩＬ１β合成量明显高于未刺激组，且铜仿膜孵育后的细胞明显高于聚砜膜和对照组。结论：正常人单个核细胞ＩＬ１β合成需双信号诱导，在尿毒症患者中内毒素和透析膜对单个核细胞ＩＬ１合成有协同作用。
OBJECTIVE ToinvestigatetheeffectofLPSanddialyzermembraneoninterleukin1(IL1)geneexpressionandproteinproductioninuremicpatientsoncontinuousambulatoryperitonaldialysis(CAPD),patientsonregularhemodialysis(HD)andhealthycontrols. METHODOLOGY IL1productionbyculturedperipheralbloodmononuclearcells(PBMC)afterexposuretocuprammonium(Cup)membrane,polysulfone(PS)membranesorendotoxinwasdetectedusingELISA.IL1mRNAexpressionwasdeterminedsimultaneouslybyinsituhybridization. RESULTS Intheabsenceofendotoxin,asmallamountofIL1wasproducedbyPBMCharvestedformHDandCAPDpatientsafterincubationwithCuporPSduringsubsequent24hourculture.Forhealthycontrols,IL1wasbarelydetectablejustabovethedetectionlimit.AlthoughnodifferenceinproteinsynthesiscouldbefoundbetweenCupandPStreatment,insituhybridizationshowedthatCupinducedmarkedlyhigherlevelofIL1mRNA.Incontrast,whensubsequentlystimulatedwithPLS,PBMCincubatedwithCubcouldproducesignificantlyhigheramountofIL1comparedwitheithernotstimulatedcellsorPBMCincubatedwithPS. CONCLUSION Twost
出处
《肾脏病与透析肾移植杂志》
CAS
CSCD
1999年第3期219-222,共4页
Chinese Journal of Nephrology,Dialysis & Transplantation
基金
上海市卫生局"百人计划"资助
关键词
透析膜
内毒素
白细胞介素-1
尿毒症
血液透析
dialyzermembraneendotoxinIL1 epsarerequiredinhealthycontrolforIL1production:inductionofmRNAtranscriptionbymembranecontact
followedbyLPSinducedtranslation
whileinuremicpatientsonHDorCAPDbioincompatibilitymembraneandLPShaveasynergeticeffectonILproduction.
