CGRP对高血压病患者动脉VSMC的NOS活性和NO合成的影响

The effects of CGRP on NOS activity and NO synthesis of VSMCs in essential hypertensive patients

目的：为探讨降钙素基因相关肽（ＣＧＲＰ）对高血压病（ＥＨ）患者动脉血管平滑肌细胞（ＶＳＭＣ）的一氧化氮合酶（ＮＯＳ）和一氧化氮（ＮＯ）产生的作用。方法：对ＥＨ患者和血压正常者（ＮＴ）的动脉ＶＳＭＣ进行培养，分别测定ＥＨ组和ＮＴ组ＶＳＭＣ的ＮＯ含量和ＮＯＳ活性，观察ＥＨ患者ＶＳＭＣＮＯ的产生以及ＣＧＲＰ对其的影响。结果：①基础状态下，ＥＨ组ＶＳＭＣ的ＮＯ含量和ＮＯＳ活性分别为４２．７３±６．７６μｍｏｌ／（２．５×１０６ｃｅｌｓ）和０．２４±０．０５ｎｍｏｌ／（２．５×１０６ｃｅｌｓ），均显著低于ＮＴ组的７４．５２±４．３７μｍｏｌ／（２．５×１０６ｃｅｌｓ）和０．５５±０．１０ｎｍｏｌ／（２．５×１０６ｃｅｌｓ）（Ｐ＜０．０５）；②在ＣＧＲＰ作用下，ＥＨ组的ＮＯ含量和ＮＯＳ活性分别为８８．６３±４．１２μｍｏｌ／（２．５×１０６ｃｅｌｓ）和１０．５５±１．２８ｎｍｏｌ／（２．５×１０６ｃｅｌｓ），均显著低于ＮＴ组的１００．４９±９．６９μｍｏｌ／（２．５×１０６ｃｅｌｓ）和１２．４５±１．６１ｎｍｏｌ／（２．５×１０６ｃｅｌｓ）（Ｐ＜０．０５）；③基础状态和ＣＧＲＰ作用下两组ＶＳＭＣ的ＮＯ含量和ＮＯＳ活性随?

Aim: To investigate the effects of calcitonin gene related peptide(CGRP) on the nitric oxide synthase (NOS) activity and nitric oxide(NO)production of vascular smooth muscle cells (VSMCs) in essential hypertensive(EH)patients Methods: The VSMCs of EH patients and the normotensives (NT) were cultured We measured the NO contents and NOS activities of the VSMC in the EH and NT groups separately, observed the NO production of the VSMCs in the EH patients and the effect of CGRP on it Results:Under basal conditions, the NO content and NOS activity of the VSMCs in the EH group were 4273676 mol/25106 cells and 024005 nmol/25106cells respectively, which were significantly lower than 7452 437 mol/25106 cells and 055010 nmol/25106cells of the NT group(P<005); (2)With the effect of CGRP, the NO content and NOS activity of the EH group were 8863 412 mol/25106 cells and 1055128 nmol/25106cells respectively, which were remarkably lower than 10049969 mol/25106 cells and 1245 161 nmol/25106celnitric oxide nitric oxide synthase vascular smooth muscle cells ls (P<005) of the NT group;Under basal conditions and with the effect of CGRP, the NO contents and NOS activities of the two groups significantly increased with the prolongation of culture time(P<005);(4)With the effect of CGRP, the NO content and NOS activities of both groups increased and enhanced significantly as compared with those under basal conditions(P<001) Conclusion: The NOS activity and NO content reduced in VSMC of the EH patients,and CGRP could enhance the activity of NOS and increase the content of NO, suggesting that the deficiency of CGRP might be involved in the pathogenesis of EH