摘要
目的观察EB病毒(EBV)原发感染后裂解期和潜伏期病毒抗原肽特异性T细胞功能变化,分析外周血EBV载量(VL)与特异性T细胞反应间的关系。方法采集首都医科大学附属北京儿童医院2006年6月至2006年7月收住院的6例EBV相关性传染性单核细胞增多症(EBV-associated IM)患儿和4例保健门诊EBV血清学抗体转化阳性的健康对照组患儿的外周血。利用磁珠分离法(MACS)检测HLA型别。在4个时间点,利用酶联免疫斑点检测法(ELISPOT)及荧光定量PCR法检测特异性T细胞分泌γ-干扰素(INF-γ)水平和外周血EBV VL。结果观察组6例特异性T细胞分泌INF-γ水平高于对照组(P<0.05)。观察组病例病程2个月时裂解期肽BMLF1特异性T细胞分泌INF-γ水平较感染急性期时下降,而潜伏期肽LMP2的结果呈上升趋势,在病程10个月时两者均较前下降,裂解肽特异性T细胞反应的下降幅度大于潜伏期肽特异性T细胞,且其水平较感染急性期和病程2个月时差异有统计学意义(P<0.05);病程20个月与病程10个月结果差异无统计学意义(P>0.05)。观察组4例(4/6)患儿外周血EBV VL为阴性。4个时间点均可检测到HLA-A11限制性BMLF1特异性T细胞分泌INF-γ阳性细胞。结论 EBV原发感染后裂解期与潜伏期肽特异性T细胞反应的变化不同,外周血EBV VL与特异性T细胞反应可能无关。HLA限制性BMLF1特异性T细胞可能在EBV原发感染后起保护作用。
Objectives To study the function of the EBV lytic and latent peptides specific CTLs and analyze the correlations between peripheral EBV Viral Load (VL) and the CTLs responses from primary to persistent infection. Methods Six patients with infectious mononucleosis ( IM ) were enrolled in the study along with four healthy children with past EBV infection as controls. Human leukocyte antigen (HLA) identification was performed by magnetic beads cell sorting (MACS). Enzyme-linked immunospot (ELISPOT) and Real-time PCR were performed. To determine INF- ~/ lever secreted by specific: T-lymphoayte & the peripheral EBV VL. Results ELISPOT and EBV VL in peripheral blood were performed in acute phase, 2 months, 10 months and 20 months after admission for all the cases except one case who lost of follow-up in 20 months. The CTLs responses against lytic peptides and latent peptides in patients were significantly in- creased (P 〈 0.05, compared with control). Among patients with IM the CTLs responses against lyric peptides decayed in contrast against latent peptides increased first then decayed from primary, to persistent infection. All of them decreased sharply in t0 months after admission (P 〈 0.05, compared with 2 months ).Then they were at a low level or undetectable ultimately (P 〉 0.05, compare 20 months with 10 months). The results of EBV VL in peripheral blood of case No. 4 were 3.52 × 10 3, 6.75× 10 2, 2× 10 2 and negative in the acute phase, 2 months, 10 months and 20 months respectively, and the results of case No. 6 were 2 × 10 3 in the acute phase and negative in other time. Four cases in the rest had negative resuits of EBV VL in peripheral blood all the time. The CTLs response against HLA restricted lytic peptide BMLF1 was positive after primary to persistent infection. Conclusions There are differences between the CTLs responses against the lytic peptides and the latent peptides and no correlations between EBV VL in peripheral blood and the CTLs responses. The abundant result of the distribution of CTLs response against BMLF1 may play a protective role after primary infection.
出处
《中国实用儿科杂志》
CSCD
北大核心
2010年第10期774-777,共4页
Chinese Journal of Practical Pediatrics
基金
北京市自然科学基金(303-04-071-147)