黄芩苷、黄芩素抑制铜绿假单胞菌生物膜形成的研究

Study on Inhibition Effect of Baicalin and Baicalein on the Formation of Pseudomonas Aeruginosa Biofilm

目的:研究黄芩苷、黄芩素对铜绿假单胞菌生物膜形成的影响。方法:选用铜绿假单胞菌X140为研究对象,用32μg·mL-1的黄芩苷、2μg·mL-1的黄芩素干预X140生物膜的培养。以阿奇霉素为对照,采用倍比稀释法对生物膜内活菌菌落计数。扫描电子显微镜(SEM)观察不同培养条件下生物膜的变化。结果:32μg·mL-1的黄芩苷、2μg·mL-1的黄芩素可以显著抑制铜绿假单胞菌的黏附性和抑制生物膜的形成(P<0.01)。结论:黄芩苷、黄芩素可抑制细菌生物膜的形成,影响铜绿假单胞菌的黏附性。

OBJECTIVE：To investigate the destructive effect of baicalin and baicalein on the formation of pseudomonas aeruginosa biofilm.METHODS：X140,a pseudomonas aeruginosa,was selected for investigation.After the biofilm was treated by baicalin or baicalein with the concentration of 32 μg·mL^-1 and 2 μg·mL^-1,the numbers of viable bacteria were measured by serial dilution using azithromycin as control.The variations were observed under scanning electronic microscope（SEM）.RESULTS：Both of the baicalin and baicalein with the concentration of 32 μg·mL-1 and 2 μg·mL^-1 could inhibit the adherence of pseudomonas aeruginosa significantly and the formation of biofilm（P〈0.01）.CONCLUSION：Baicalin and baicalein can inhibit the adherence of pseudomonas aeruginosa and the formation of biofilm.