生姜醇提取物对人肺腺癌细胞(A549)增殖和凋亡的影响

Effects of the Ethanol Abstracts of Zingiber officinale on the Proliferation and Apoptosis of Human Lung Adenocarcinoma Cell(A549)

目的:研究生姜醇提取物对人肺腺癌细胞增殖和凋亡的影响。方法:用浓度为10、20、40μmol·L-1生姜醇提取物分别处理A549细胞24h,以未经生姜醇提取物处理的A549细胞为对照。MTT比色法检测A549细胞增殖能力;光镜观察细胞的生长情况、形态学改变;原位末端TdT酶标记技术(TUNNEL)检测细胞凋亡的情况。结果:MTT比色法结果表明,10、20、40μmol·L-1生姜醇提取物抑制率分别为14.87%、24.72%、38.21%,与对照组(0.04%)比较抑制率显著增加(P<0.01),并随生姜醇提取物浓度增高而显著增加;光镜观察对照组细胞生长正常,凋亡细胞少见,而生姜醇提取物处理的A549细胞生长疏散,凋亡细胞的比例增加,程度随生姜醇提取物浓度增高而加重,尤以40μmol·L-1作用24h最显著;TUNNEL检测的10、20、40μmol·L-1生姜醇提取物下的A549细胞凋亡细胞指数分别为(9.36±0.82)%、(13.74±1.15)%、(19.17±1.40)%,与对照组(4.81±0.36%)比较均有显著差异(P<0.01),并随生姜醇提取物浓度增高而显著增加。结论:生姜醇提取物能抑制人肺腺癌细胞的增殖,其作用呈明显剂量依赖性,即随剂量的增加而抑制率增加。

NG Lin,WU Qing-chen,ZHANG Cheng（Dept.of Cardiothoracic Surgery,The First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China） Abstract：OBJECTIVE：To investigate the effects of the ethanol extracts of Zingiber officinale on the proliferation and apoptosis of human lung adenocarcinoma cell（A549）.METHODS：The ethanol extracts（10 μmol·L^-1,20 μmol·L^-1,40 μmol·L^-1） of Z.officinale were interacted with A549 cell line for 24 h with untreated A549 cells as control.The proliferation capacity of A549 cells was detected by MTT method.The growth and morphological changes of A549 cells were observed by light microscope.Terminally labeled transferase technique（TUNEL） apoptosis of A549 cells were detected.RESULTS：Results of MTT assay showed that the inhibition rates of cell proliferation were 14.87% at 10 μmol·L-1 ethanol extracts of Z.officinale,24.72% at 20 μmol·L^-1 ethanol extracts of Z.officinale and 38.21% at 40 μmol·L-1 ethanol extracts of Z.officinale.The inhibition rates of experiment group were higher than that of control group（0.04%） significantly（P〈0.01）;the inhibition rate increased as the ethanol extracts concentration of Z.officinale increased.The results of light microscopy showed few apoptotic cells in control group.There were scattered A549 cells and more apoptotic cells in experiment group as the ethanol extracts concentration of Z.officinale increased,especially with a dose of 40 μmol·L-1 for 24 h.TUNNEL test showed the index of apoptotic cells was（9.36±0.82）%,（13.74±1.15）%,（19.17±1.40）% in experiment group,which were significantly different from（4.81±0.36%） of control group（P〈0.01）.It also increased significantly as the ethanol extracts concentration of Z.officinale increased.CONCLUSION：The ethanol extracts of Z.officinale could inhibit the proliferation of A549 cell line in dose-dependent manner.In another word,inhibition rate of ethanol extracts increased as the concentration of ethanol extracts increased.