鱼精蛋白凝聚法测定和厚朴酚脂质体的包封率

Determination of Encapsulation Efficiency of Honokiol Lipsomes by Protamine Aggregation Method

目的:建立和厚朴酚脂质体包封率(EE)的测定方法。方法:以反相高效液相色谱法为分析手段,采用鱼精蛋白凝聚法测定和厚朴酚脂质体EE。结果:和厚朴酚检测浓度在2.006～160.512mg·L-1范围内与峰面积积分值呈良好的线性关系(r=0.9999),最低检测限和最低定量限分别为1.4、4.0ng;鱼精蛋白凝聚法的平均回收率为(95.70±2.07)%,RSD=1.71%(n=9);平均EE为85.87%。结论:鱼精蛋白凝聚法操作简单、可行、重现性好,适用于脂溶性药物和厚朴酚脂质体EE的测定。

OBJECTIVE：To establish a method for the determination of encapsulation efficiency（EE） of honokiol liposomes.METHODS：RP-HPLC method was adopted for the analysis of honokiol liposomes.Protamine aggregation method was applied to determinate the encapsulation efficiency of honokiol liposomes.RESULTS：The linear range of honokiol was 2.006～160.512 mg·L^-1.The limits of detection and quantitation were 1.4 ng and 4.0 ng,respectively.The mean recovery rate of protamine aggregation method was（95.70±2.07）%（RSD=1.71%,n=9）.The mean encapsulation efficiency of honokiol liposomes was 85.87%.CONCLUSION：The protamine aggregation method is simple,practical and reproducible for determination of the encapsulation efficiency of honokiol liposomes.