下颌骨节段性缺损修复组织工程中珊瑚支架残留率的研究

Scaffold residue proportion in coral-engineered bone repairing segmental mandibular defects

目的探讨骨髓基质干细胞(bone marrow stromal cell,BMSC)复合珊瑚修复犬下颌骨节段性缺损支架的残留率。方法体外扩增、成骨诱导培养犬BMSC,分别将第二代细胞复合珊瑚后植入犬自体右侧3cm的下颌骨节段性缺损,术后12周(n=6),32周(n=6)取材后分别通过Micro-CT检测和大体、组织学图像分析骨修复、支架残留率和生物力学修复强度。结果 Micro-CT检测和组织学图像分析均表明,BMSC-珊瑚组组织工程骨12周时支架残留率显著高于32周(P<0.05),而新骨随材料降解逐步成熟;生物力学显示术后32周力学强度显著高于12周(P<0.05)。结论自体成骨诱导BMSC复合珊瑚形成的组织工程骨可良好修复犬下颌骨节段性缺损,随时间延长材料逐步降解,组织工程下颌骨进一步成熟。

Objective To analyze the residual material volume （RMV） in repair of canine segmental mandibular defects with coral-engineered bone [osteogenic induced bone marrow stromal cells （BMSC）-coral].Methods Isolated BMSC were in vitro expanded and osteogenic. In 12 canines, a 3 cm segmental mandibular defect at right mandible was created, the defects were repaired with BMSC-coral constructs. 6 canines were sacrificed at 12 weeks and 32 weeks, respectively. The engineered bone was evaluated by Micro-CT, gross and histomorphometry examination, and biomechanical analysis.Results Micro-CT and histomorphometry examination both revealed the RMV of coral-engineered bone at 12 weeks was significantly higher than at 32 weeks post-operatively （P 〈 0.05）, and the new bone matured gradually during the scaffold degradation. The biomechanical strength of engineered bone at 12 weeks was significantly lower than at 32 weeks （P 〈 0.05）.Conclusions Canine segmental mandibular defects could be well repaired by osteogenic induced BMSC-coral engineered bone, the engineered bone further matured synchronized scaffold resorption.