IL-27对哮喘小鼠气道炎症的影响

Influence of IL-27 on airway inflammation of asthmatic guinea pigs

目的研究IL-27对卵白蛋白(OVA)激发哮喘小鼠气道炎症的影响。方法 24只雌性BALB/c小鼠随机分为生理盐水组、哮喘组及IL-27组,每组8只。应用OVA建立哮喘模型,IL-27组小鼠应用1μgIL-27(溶于50μlPBS中)滴鼻给药,观察3组小鼠肺组织病理改变,计数支气管肺泡灌洗液(BALF)中嗜酸性粒细胞;ELISA法测定小鼠BALF中IL-4和IFN-γ浓度,RT-PCR测定肺组织T-bet mRNA的表达量。结果 IL-27组小鼠肺组织炎症反应明显轻于哮喘组小鼠;IL-27组小鼠BALF中嗜酸性粒细胞计数为(2.21±0.33)×107/L明显低于哮喘组的(12.82±2.17)×107/L(P<0.01);IL-27组小鼠BALF中IL-4浓度为(20.4±3.2)μg/L,明显低于哮喘组的(61.3±13.1)μg/L(P<0.05);IL-27组小鼠BALF中IFN-γ浓度为(50.3±6.3)μg/L,明显高于哮喘组的(11.1±3.3)μg/L(P<0.05);IL-27组小鼠肺组织T-bet mRNA表达量(吸光度积分比值)为(0.268±0.048),明显高于哮喘组的(0.130±0.012)(P<0.05)。结论 IL-27可能通过增强T-bet mRNA的表达增强Th1反应,减少BALF中嗜酸性粒细胞数量,进而减轻了哮喘小鼠肺组织炎症反应。

Objective To study the influence of IL-27 on airway inflammation of asthmatic guinea pigs induced by ovalburnin （OVA）. Methods 24 female BALB/c mice were randomly divided into saline group, asthmatic model group and IL-27 group with each group of 8 ones. Asthmatic model was established with the application of OVA, 1 μg IL-27 dissolved in 50 μl PBS was administered intranasally to IL-27 group, and then lung histopathological change was observed and eosinophils in bronchoalveolar lavage fluid （BALF） were counted in 3 groups; IL-4 and IFN-γin BALF were detected with ELISA, expression of T-bet mRNA in lung tissue was detected with RT-PCR.Results Inflammatory response in lung tissue of IL-27 group was less than that of asthmatic model group; compared with those in asthmatic model group, eosinophil counting in BALF was significantly decreased [（2.21±0.33）× 107/L vs （12.82 ±2.17） × 107/L, P 〈 0.01]; IL-4 concentration in BALF was significantly decreased [（20.4 ± 3.2） μg/L vs （61.3 ±13.1） μg/L, P 〈 0.05）] while IFN-γ concentration in BALF was significantly increased [（50.3 ±6.3） μg/L vs （11.1 ± 3.3） μg/L, P 〈 0.05） ] and T-bet mRNA synthesis （ratio of integral absorbance） in lung tissue was significantly increased [（0.268 ± 0.048） vs （0.130 ± 0.012）, P 〈 0.05）] in IL-27 group.Conclusion IL-27 maybe strengthen Th1 response and reduce number of eosinophils in BALF so as to reduce lung tissue inflammation in asthmatic guinea pigs by increasing T-bet mRNA expression.