TRAP—PCR ELISA及银染法检测肺刷落细胞中端粒酶活性

The Detection Of Telomerase Activity in Bronchoscopic cells in Lung Cancer

以非同位素法检测肺刷落细胞中端粒酶活性及在肺癌中的诊断价值。方法采用端粒酶重复放大程序ＴＲＡＰ（ＴｅｌｏｍｅｒａｓｅＲｅｐｅａｔＡｍｐｌｉｆｉｃａｔｉｏｎＰｒｏｔｏｃｏｌ）ＰＣＲＥＬＩＳＡ法及聚丙烯酸胺凝胶电泳一银染法，对８０例支气管肺刷落细胞样本进行定性及定量端粒酶活性检测。结果肺癌患者患侧端粒酶定性检测阳性率为８８．６％，其对侧部位为２２．７％，肺炎阳性率为１１．８％。定量法检测肺癌病人的端粒酶活性水平（中位ＯＤ值：０．０８９）明显高于其对侧肺部（中位ＯＤ值：０．０２８）及肺炎组（中位ＯＤ值：０．０１４）。另外病理发现不典型增生者６例中有５例端粒酶阳性。结论应用该方法对肺刷落细胞中端粒酶活性的测定可以作为肺部恶性肿瘤诊断有效而敏感的手段之一。

Objective :To investigate the diagnostic siedcance of the detection of telomerase activity in the exfoliated cells obtained from fiberobronchoscopic brushing.Methods TRAP-PCR-ELISA and TRAP-Silver Staining were employed to detecttelomerase activity in 80 cases Of pulmonaly diseases. ResultS Furthermore, the positive rate of telomerase achvity detection in lung cancer patients (88.6%)were significantely higher than that in pneumona (11.8%).The telomerase activity levelsin the lesion side of lung cancer patients (median 0.089)are Obviously higher than that in the other side of same patient(median 0.028) (P>0.0l),and that in pneumonia (median 0.014) (P<0.01). And 5 of 6 cases with cytological dysplasia werefound telomerase activity Positive(median 0.074). Conclusion These findings suggested that the detection of telomerase activity in the exfoliated cells obtained from fiberobronchoscopic brushing might serve as an effective and sensitive method inthe diagnose of pulmonary malignant diseases.