雷公藤多甙对大鼠精子凋亡的影响

Multiglycosides of tripterygium wilfordii increase sperm apoptosis in male rats

目的:研究雷公藤多甙(GTW)对大鼠精子凋亡的影响。方法:将SD雄性大鼠16只均分成2组,雷公藤多甙组和羧甲基纤维素钠对照组均为剂量20 mg/(kg.d)灌胃,6周后取大鼠附睾精子测定其凋亡率、膜脂流动性、一氧化氮(NO)、丙二醛(MDA)及超氧化物歧化酶(SOD)含量的变化。结果:灌胃6周后,GTW组精子的凋亡率、NO和MDA的含量显著增加(与对照组相比,P<0.01),精子膜脂流动性(P<0.05)和SOD含量(P<0.01)降低。结论:雷公藤多甙可致大鼠精子膜脂质过氧化损伤、膜结构受损以及膜流动性下降,精子凋亡率升高。

Objective：To study the effect of multiglycosides of tripterygium wilfordii（GTW） on sperm apoptosis in male rats and its possible mechanisms.Methods：Sixteen male SD rats were equally assigned to two groups to receive GTW and carboxymethylcellulose（CMC） intragastrically,both at 20 mg /（kg·d） for 6 weeks.Then the epididymal sperm was collected for the measurement of the apoptosis rate,sperm membrane lipid fluidity and the contents of NO,MDA and SOD by flow cytometry and spectrophotometric determination.Results：After 6 weeks of medication,the GTW group showed a significant increase in sperm apoptosis and contents of NO and MDA（P〈0.01） and a remarkable decrease in sperm membrane lipid fluidity（P〈0.05） and SOD content（P〈0.01） as compared with the CMC control group.Conclusion：GTW can damage sperm membrane lipid peroxidation and sperm membrane structure,increase sperm apoptosis,and reduce sperm membrane lipid fluidity.