腺苷对兔肺缺血再灌注损伤的保护作用

Protective effects of adenosine against lung ischemia-reperfusion injury in rabbit

目的 探讨腺苷（Adenosine）对兔肺缺血再灌注损伤的保护作用及可能机制.方法 建立兔肺缺血再灌注动物模型.健康家兔30只,随机分为3组：A组为假手术组、B组为缺血再灌注组、C组为腺苷处理＋缺血再灌注组,每组10只,每组分别于再灌注60 min时测定血浆丙二醛（MDA）、超氧化物歧化酶（SOD）、肺组织湿干重比（W/D）,光镜观察肺组织形态结构变化并测定肺损伤定量评价指标（IQA）,流式细胞仪测定CD11b/CD18-FTTC标记的中性粒细胞（PMN）阳性细胞百分率.结果 再灌注60 min后,与A组相比,B组W/D、血浆MDA含量以及IQA显著升高（q=7.06,13.71,18.62,P＜0.01）,而SOD含量显著降低（q=14.33,P＜0.01）.C组W/D、血浆MDA含量以及IQA较B组明显降低（q=5.23,8.51,9.99,P＜0.01）,而SOD含量则明显升高（q=7.73,P＜0.01）.B组CD11b/CD18-FITC标记的PMN阳性细胞百分率明显高于A组及C组（q=8.59,9.56,P＜0.01）.结论 腺苷可能通过抑制PMN表面CD11b/CD18表达的上调,减少氧自由基的生成,对肺缺血再灌注损伤产生保护作用.

Objective To study the protective effects and mechanisms of adenosine on lung ischemia-reperfusion injury in rabbit. Methods The rabbit ltng model of ischemia-reperfusion was constructed.Thirty Chinese rabbits were random divided into three groups： Group A （no surgery）, group B （ischemiareperfusion） and group C （Adenosine ＋ ischemia-reperfusion）. The MDA content,SOD content of the plasma, wet-dryrate （W/D） and the pathology of lung tissue and the index of quantitative assessment of histologic lung injury （IQA） were measured after 60 min reperfusion. Results After 60min reperfusion, the value of W/D, MDA and IQA in group B were significantly higher than those in group A （q = 7. 06,13.71,18. 62, P 〈0.01）, while the concentration of SOD were lower than those in group A （q = 14. 33, P 〈0.01）. In contrast with group B, W/D,MDA and IQA in group C was obviously lower （q =5.23 ,8. 51, 9.99,however, the concentration of SOD were higher than those in group B （q = 7.73, P 〈 0. 01）. In contrast with group A and C ,the expression of CD11b/CD 18 of group B was significantly increased after 60min reperfusion （q =8.59,9.56, P 〈0. 01）. Conclusion Adenosine can prevent ischemia-reperfusion injury in rabbit lung in vivo by inhibiting the expression of CD-11b/CD18 on PMNs and dropping oxygen free radicals level.