微刻沟嵴钛喷涂硅片对成骨细胞形态与功能的影响

Effects of Micromachined Grooved Ticoated Surface on Osteoblast Shape and function

许多研究证明种植体表面形态可以改变细胞的性质和功能。研究主要集中于上皮细胞和成纤维细胞。对于与种植体骨性愈合紧密相关的成骨细胞研究较少。本实验应用微刻沟嵴钛喷涂硅片，利用共焦激光扫描显微镜（ＣＬＳＭ）观察成骨细胞外形和细胞骨架结构的变化。实验结果显示，实验组细胞伸展方向同沟嵴方向一致。细胞骨架结构微丝、微管和纽带蛋白的分布，在沟嵴表面表现出明显的按沟嵴方向走行。相信应用更复杂结构的材料，可引导细胞按人们预先设计的方向生长，最终可形成理想种植体界面。

urface topography varies widely among commercially available dental implants.In order to understand cell behavior during osseointegration some researchers have investigated epithelial cell and fibroblast.In this experiment,we used micromachined grooved ticoated substrata to investigate the shape and cytoskeleton distribution of osteoblast harvested from rat calvaria. CLSM, immunofluorescence and image analyses were used to measure cell orientation angle and Form Ell and cytoskeleton distribution (Microfilament,Microtuble and Vinculin).Compared with smooth surfaces,grooved surfaces influenced the cell shape and cytoskeleton distribution,which were alingned with the grooves.It was the same as what were found in epithelial cell and fibroblast before.We could conclude that desired osseointegration of dental implants would be achieved by using more complex topographic materials to guide cells spreading and locomotion.