KC和FSC对肝细胞增殖及合成功能的影响

The Effect of KC and FSC to Hepatocytes Proliferation and Synthesis Function

目的在于观察体外培养条件下，库普弗细胞（ＫＣ）贮脂细胞（ＦＳＣ）对肝细胞（ＰＣ）基质生成调节效应及ＰＣ增殖及合成功能的影响。采用ＦＳＣ、ＫＣ和ＰＣ的分离培养，观察ＫＣ和ＦＳＣ对ＰＣ增殖活性和ＤＮＡ含量的影响，应用免疫细胞化学和图像分析系统对ＰＣ中Ⅰ、Ⅱ、Ⅳ型胶原和纤维粘连蛋白（ＦＮ）进行定量分析，以及用液闪法检测ＰＣ中３Ｈ－脯氨酸掺入量。结果证明ＦＳＣｎｃｍ可明显促进ＰＣＤＮＡ合成，ＰＣ可合成Ⅰ、Ⅲ、Ⅳ型胶原及ＦＮ。ＦＳＣｎｃｍ可促使双核的ＰＣ增多，线粒体分裂。提示肝细胞可合成Ⅰ、Ⅲ、Ⅳ型胶原及ＦＮ；ＰＣ内可能直接组装胶原纤维；ＫＣｎｃｍ、ＫＣｔｃｍ、ＦＳＣｔｃｍ均可上调ＰＣ的基质合成能力。

J We observed that effect of Kupffer Cells(KC) and Fat Store Cells(FSC) to liver parenchymal cells(PC) matrix svnthesis regulating and PC proliferation and function. We adopted separation cultured of FSCKC and PC,observed effect of KC and FSC to proliferation activity and DNA content of PC. It used immunocytochemistry and image pattern analysis system to quantitative analysis collagen type and fibnectin(FN) in PC,and applied liquid scintillation method to detecting incorporation number of 3Hprolin in PC.FSCncm can promote obviously PC DNA synthesis.PC can synthesis collagen type and FN.FSCncm may impel two nucleus PC increasing and mitochondria cleavage.The hepatocytes can synthesis collagen type and FN,It may directly compose collagen fiber in PC,All of KCncmKCtcmFSCtcm can promote PC synthesis ability of matrix.