瓜蒌薤白半夏汤舒张血管机制研究

Relaxant effect of Gualouxiebaibanxia Decoction on rat aortic rings

目的:观察瓜蒌薤白半夏汤(GXB)的血管舒张作用及其机制。方法:采用大鼠离体血管功能实验装置,描记张力变化,每组血管来自6只同批大鼠,分别保留或去除内皮,各阻断剂预处理25min之后做GXB舒张曲线。结果:GXB(1μg/ml～1000μg/ml)剂量依赖性地舒张苯肾上腺素(PE)预收缩的内皮完整或去除内皮的大鼠胸主动脉环,且舒张效应在有无内皮血管之间差异显著(P<0.01),内皮去除前后最大舒张效应Emax分别为77.4%±7.9%和34.8%±4.6%,EC50由完整内皮的462.5μg/ml到去除内皮的1630.8μg/ml(P<0.01);与对照组比较,一氧化氮合酶抑制剂L-NAME0.1mmol/L、鸟甘酸环化酶抑制剂ODQ0.01mmol/L,环氧合酶抑制剂indomethacin0.01mmol/L预处理可抑制GXB对内皮完好血管的舒张效应(P<0.01),最大舒张效应Emax分别为L-NAME组56.7%±5.6%,ODQ组50.6%±1.7%,indomethacin组66.1%±9.5%,EC50分别为L-NAME组789.9μg/ml,ODQ940.9μg/ml,indomethacin组719.8μg/ml。结论:GXB具有舒张血管作用且为内皮依赖性舒张,其机制主要与内皮NO-cGMP途径和环氧合酶途径有关。

Objective：To investigate the mechanism underlay the vasodilatory effect of Gualouxiebaibanxia Decoction（GXB）.Methods：This study was performed with the isolated rat aortic rings in organ bath,and the tension changes of aortic rings were recorded.the rings of each group were from 6 rats,and with endothelium intact and removed respectively,some groups were pretreated with different inhibitors for 25min,and then make the GXB relaxantion.Result：GXB（1μg/ml～1000μg/ml） concentration-dependently relaxed isolated aorta ring,and the maximum relaxation by GXB was 77.4%±7.9% in endothelium intact rings and 34.8%±4.6% in endothelium removed rings while the 1 μmol/L forskolin-induced vasodilation was took as 100%.EC50 for GXB-induced vasodilation was 462.5μg/ml in endothelium intact group and 1630.8μg/mlin endothelium removed groups（P〈0.01）.Compared to control group,the vasodilatory effect by GXB on endothelium intact groups were significantly inhibited after 0.1 mmol/L L-NAME,0.01 mmol/L ODQ and 0.01mmol/L indomethacin incubated for 25min（P〈0.01）,their Emax was 56.7%±5.6%,50.6%±1.7%,66.1%±9.5%,and EC50 was 789.9μg/ml,940.9μg/ml,719.8μg/ml in each group respectively.Conclusion：The GXB have the effect of vasorelaxation and the mechanism maybe through NO-cGMP and cycloxygenase signal pathway.