脑舒胶囊对阿尔茨海默病大鼠学习记忆力的改善及海马神经元的保护

The improvement of Naoshu Capsule on capability of learning and memory and the propection to hippocampus neurons of AD Rat

目的:研究脑舒胶囊对阿尔茨海默病模型大鼠的防治效果及其作用机理。方法:双侧脑室注射Aβ25-35复制阿尔茨海默病大鼠模型;Morris水迷宫观察大鼠学习记忆能力;免疫组织化学法(SABC法)检测大鼠海马Bcl-2、Bax及caspase-3的表达;HE染色光镜观察海马神经元损伤情况。结果:与假手术组比较,模型组大鼠的逃避潜伏期明显延长,跨越虚拟平台的次数及在平台象限停留的时间明显减少;Bcl-2和Bax表达均有所增加,其平均光密度比值(Bcl-2/Bax)明显下降;Caspase-3阳性表达明显增高。与模型组比较,0.72mg/kg和1.44mg/kg脑舒胶囊可缩短大鼠逃避潜伏期,增加大鼠跨越虚拟平台的次数及在平台象限停留的时间;使Bcl-2阳性表达明显所增加,Bax阳性表达明显减少,升高Bcl-2/Bax值;明显降低Caspase-3的阳性表达;光镜观察发现模型大鼠海马神经元排列较紊乱、数量减少、体积变小,胞浆浓缩,胞核固缩深染,结构不清,各剂量的脑舒胶囊均能在一定程度上改善上述神经元损伤。结论:脑舒胶囊可改善阿尔茨海默病大鼠的记忆能力及Aβ25-35所致海马神经元损伤,其原因与其提高抗凋亡基因Bcl-2的表达,抑制促凋亡基因Bax表达和caspase-3表达,进而抑制海马神经元的凋亡有关。

Objective：To investigate the preventive and therapeutic effect of Naoshu Capsule on AD rats and the mechanism.Methods：AD model was induced by injecting Aβ25-35 in the bilateral lateral ventricles of rat,learning and memory capability of rats was observed with Morris water maze,expression of Bcl-2,Bax and caspases-3 in hippocampus was detected by SABC,hippocampal neuron injuries was observed by HE staining.Results：Compare with sham operation group,the rats in model group show longer escape latency,and less times to cross the virtual platform and staying on the platform,the expression of Bcl-2 and Bax strengthens,the ratio of their optical density（Bcl-2/Bax） obviously decreased,the positive expression of Caspase-3 obviously enhanced.Compare with the model group,in the 0.72mg/kg and 1.44mg/kg Naoshu Capsule group,there is shorter escape latency of rats,more times to cross the virtual platform and longer tine to stay on the platform,strenthen the positive expression of Bcl-2,inhibit the positive expression of Bax,increase the ratio of their optical density（Bcl-2/Bax） ;decrease the positive expression of Caspase-3;and in the light microscope,the hippocampus neurons in the model group have irregular arrange ment,samll number,small volume,thick plasm,pycnotic and thick nucleus,blur structure;the improvement occur in all the different Naoshu Capsule Group.Conclusion：Naoshu Capsule can improve AD rats＇memory amd alleviate neurons＇damage by Aβ25-35.The mechanism may be that the Naoshu Capsule increased the expression of Bcl-2,inhibited the expression of Bax and caspase-3,finally inhibit the apotisis of hippocampus neurons.