不同中医治法对帕金森病大鼠6-酮前列腺素、血栓素B_2和内皮素的影响

The influence of different therapeutic methods in TCM on ET,TXB_2 and 6-keto-PGF_(1α) of PD rats

目的研究不同中医治法对帕金森病(PD)大鼠内皮素(ET)、6-酮前列腺素F1α(6-keto-PGF1α)和血栓素B2(TXB2)的影响。方法采用经典的6-羟基多巴胺损毁注射法制作PD模型,并应用活血化瘀(代表方桃红四物汤)、涤痰熄风(代表方涤痰汤)、滋阴熄风(代表方天麻钩藤饮)和复合治法(代表方复方地黄方)等不同方剂进行治疗,同时设立正常对照组、假手术组作对照。放射免疫法测定大鼠血液ET、6-keto-PGF1α和TXB2的含量。结果模型组TXB2、ET明显升高、6-keto-PGF1α明显降低,与正常对照组和假手术组相比差异均有统计学意义(P<0.01)。与模型组相比,各药物治疗组ET差异均具有统计学意义(P<0.01);天麻钩藤饮组、复方地黄方组的三个指标差异均有统计学意义(P<0.05或P<0.01)。天麻钩藤饮组和复方地黄方组分别与桃红四物汤组比较,TXB2和6-keto-PGF1α差异均有统计学意义(P<0.05或P<0.01);天麻钩藤饮组和复方地黄方组分别与涤痰汤组比较,各指标差异均无统计学意义(P>0.05);天麻钩藤饮组和复方地黄方组比较,各指标差异均无统计学意义(P>0.05)。结论各种治法均有明显的使PD大鼠血液血管活性物质TXB2、ET和6-keto-PGF1α的部分或全部指标向正常水平恢复的作用,但以天麻钩藤饮为代表的滋阴熄风治法和以复方地黄方为代表的复合治法为好。

Objective To investigate the influence of different therapeutic methods in TCM on ET,6-keto-PGF1α and TXB2 of PD rat model. Methods The PD rat models were made by the classic damaging methods of injecting 6-OHDA. The several different kinds chinese medical formulases were applied to treat the model. The therapeutic method of Ziyin Xifeng was presented by Tianma Gouteng Decoction,the therapeutic method of Huoxue Huayu was presented by Taohong Siwu Decoction; the therapeutic method of Ditan Xifeng was presented by Ditan Decoction; the complex therapeutic method was presented by Complex Dihuang Decoction. The changes of vasoactive substances in different PD rats groups were observed,and compared with normal and sham groups. The contents of ET,6-keto-PGF1α and TXB2 in PD rats were detected by RIA. Results The levels of TXB2 and ET of PD model rats were increased,the level of 6-keto-PGF1α was decreased（P0.05,or P0.01） compared with those of normal and sham groups. After therapy and compared with those of PD model group,the levels of indicators of every therapy groups were statistically significant （P0.01）; The detected indicators of Tianma Gouteng Decoction group and Complex Dihuang Decoction group were statistically significant（P0.05或P0.01）. Compared with those of Taohong Siwu Decoction group,the levels of TXB2 and 6-keto-PGF1α were obviously statistically significant （P0.05,or P0.01）,which were detected in Tianma Gouteng Decoction and Complex Dihuang Decoction groups. Compared with those of Ditan Decoction group,all of the indictors had no significant difference in Tianma Gouteng Decoction and Complex Dihuang Decoction groups （P0.05）. Compared with those of Tianma Gouteng Decoction and Complex Dihuang Decoction groups,all of the indictors had no obvious difference （P0.05）. Conclusions Every therapeutic methods in TCM could make 6-keto-PGF1α,TXB2 and ETs＇ contents tend to the normal levels,but the complex TCM therapy and ZIYIN therapy have the best effects.