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单核细胞趋化蛋白-1对前列腺癌PC-3M细胞生长的影响 被引量：3

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摘要 目的探讨单核细胞趋化蛋白-1(MCP-1)对前列腺癌PC-3M细胞生长的影响。方法选取PC-3M细胞平铺于培养板24h,待细胞达到80%融合度时随机分为对照组、Si-scramble组、MCP-1组和Si-MCP-1组。对照组无特殊处置,MCP-1组加入MCP-150ng/ml,Si-scramble组和Si-MCP-1组分别转染pGCSi-scrambled质粒和pGCSi-mcp-1质粒,细胞处理后再培养24h,比较各组细胞迁移速率变化,72h时进行细胞抑制率检测以及应用流式细胞仪测定各组细胞凋亡率。结果细胞处理培养24h后,MCP-1组细胞迁移速率〔(13.4±3.21)μm/24h〕与对照组〔(7.3±1.24)μm/24h〕和Si-scramble组〔(5.7±1.73)μm/24h〕比较明显加快(P<0.05),而Si-MCP-1组的细胞迁移速率〔(2.1±0.92)μm/24h〕与对照组和Si-scramble组比较明显减慢(P<0.05)。72h后Si-MCP-1组细胞凋亡率可达(19.2±2.69)%,与对照组〔(5.3±0.35)%〕和Si-scramble组〔(5.4±0.35)%〕的细胞凋亡率比较凋亡率明显升高(P<0.05),MCP-1组细胞凋亡率为(3.2±0.42)%,与对照组和Si-scramble组比较凋亡率明显降低(P<0.05)。结论MCP-1对PC-3M细胞有促进增长和转移的作用,而pGCSi-MCP-1质粒可显著抑制肿瘤细胞的生长和转移。
作者 施朝龄 于长海 张羽 苗强 常喜华 王伟华
机构地区 吉林大学第四医院 吉林大学中日联谊医院泌尿外科
出处 《中国老年学杂志》 CAS CSCD 北大核心 2010年第18期2636-2637,共2页 Chinese Journal of Gerontology
关键词 前列腺癌 PC-3M 单核细胞趋化蛋白-1 RNA干扰 凋亡
分类号 R737.25 [医药卫生—肿瘤]
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参考文献10

  • 1Loberg RD,Day LL,Harwood J,et al.CCL2 is a potent regulator of prostate cancer cell migration and proliferation[J].Neoplasia,2006;8(7):578-86.
  • 2van Golen KL,Ying C,Sequeira L,et al.CCL2 induces prostate cancer transendothelial cell migration via activation of the small GTPase Rac[J].J Cell Biochem,2008;104(5):1587-97.
  • 3Loberg RD,Ying C,Craig M,et al.Targeting CCL2 with systemic delivery of neutralizing antibodies induces prostate cancer tumor regression in vivo[J].Cancer Res,2007;67(19):9417-24.
  • 4Roca H,Varsos Z,Pienta KJ.CCL2 protects prostate cancer PC3 cells from autophagic death via hosphatidylinositol 3-kinase/AKT-dependent survivin up-regulation[J].J Biol Chem,2008;283(36):25057-73.
  • 5Datta HK,Ng WF,Walker JA,et al.The cell biology of bone metabolism[J].J Clin Pathol,2008;61(5):577-87.
  • 6Keller ET,Brown J.Prostate cancer bone metastases promote both osteolytic and osteoblastic activity[J].J Cell Biochem,2004;91(4):718-29.
  • 7Dougall WC,Chaisson M.The RANK/RANKL/OPG triad in cancer-induced bone diseases[J].Cancer Metastasis Rev,2006;25(4):541-9.
  • 8Roodman GD.Mechanisms of bone metastasis[J].N Engl J Med,2004;350(16):1655-64.
  • 9Lu Y,Chen Q,Corey E,et al.Activation of MCP-1/CCR2 axis promotes prostate cancer growth in bone[J].Clin Exp Metastasis,2009;26(2):161-9.
  • 10Mizutani K,Sud S,McGregor NA,et al.The chemokine CCL2 increases prostate tumor growth and bone metastasis through macrophage and osteoclast recruitment[J].Neoplasia,2009;11(11):1235-42.

同被引文献35

  • 1CHIU H Y,SUN K H,CHEN S Y, et al. Autocrine CCL2 pro-motes cell migration and invasion via PKC activation and tyrosinephosphorylation of paxillin in bladder cancer cells[ J]. Cytokine,2012,59(2) : 423 -432.
  • 2FANG W B, JOKAR I,ZOU A, et al. CCL2/CCR2 chemokinesignaling coordinates survival and motility of breast cancer cellsthrough Smad3 protein and p42/44 mitogen - activated protein ki-nase (MAPK) - dependent mechanisms[ J]. J Biol Chem, 2012,287(43): 36593 -36608.
  • 3ZHANG J, PATEL L, PIENTA K J. Targeting chemokine (C - Cmotif) ligand 2 ( CCL2 ) as an example of translation of cancer mo-lecular biology to the clinic [ J ] . Prog Mol Biol Transl Sci, 2010 ,95: 31 -53.
  • 4ZHANG J,LU Y,PIENTA K J. Multiple roles of chemokine ( C -C motif) ligand 2 in promoting prostate cancer growth [ J ]. J NatlCancer Inst, 2010,102(8) : 522 -528.
  • 5HINGE J, STRASSBURG S,NEUMANN K, et al. Towards in si-tu tissue repair .. human mesenchymal stem cells express chemokinereceptors CXCR1,CXCR2 and CCR2,and migrate upon stimula-tion with CXCL8 but not CC12 [ J ]. J Cell Biochem, 2007,101(1); 135 -146.
  • 6VANDE B I,ASOSINGH K, VANDERKERKEN K, et al. Che-mokine receptor CCR2 is expressed by human multiple myelomacells and mediates migration to bone marrow stromal cell - pro-duced monocyte chemotactic proteins MCP - 1,- 2 and - 3 [ J ].Br J Cancer, 2003, 88(6) : 855 -862.
  • 7LU Y, CAI Z, XIAO G,et al. CCR2 expression correlates withprostate cancer progression[J]. J Cell Biochem, 2007, 101(3):676 一685.
  • 8ROCA H,VARSOS Z S, MIZUTANI K, et al. CCL2, survivinand autophagy : new links with implications in human cancer[ J].Autophagy, 2008, 4(7): 969-971.
  • 9TSAI W H, SHIH C H, LIN C C, et al. Monocyte chemotacticprotein -1 in the migration of differentiated leukaemic cells towardalveolar epithelial cells[ J]. Eur Respir J,2008,31(5) : 957 -962.
  • 10DE VASCONCELLOS J F, LARA顺IRA A B,ZANCHIN N I, etal. Increased CCL2 and IL - 8 in the bone marrow microenviron-ment in acute lymphoblastic leukemia [ J ]. Pediatr Blood Cancer,2011,56(4): 568 -577.

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中国老年学杂志
2010年 第18期

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