影响牙种植体早期边缘骨吸收的相关因素的临床初步研究

The relationship between IL-1RN gene polymorphism and marginal bone loss around dental implants

目的:探讨白细胞介素-1受体拮抗剂基因(IL-1RN)多态性是否与种植体Ⅱ期手术前边缘骨吸收有关。方法:采用病例对照研究,选取44例种植患者根据有无边缘骨吸收分为病例组和对照组;同时收集所有患者的颊黏膜拭子提取DNA;采用聚合酶链式反应测定IL-1RN的基因型。结果:携带IL-1RNⅠ/Ⅱ基因型的患者早期边缘骨吸收的发生率明显高于Ⅰ/Ⅰ基因型和Ⅰ/Ⅳ基因型的患者。据多元logistic回归分析,IL-1RNⅠ/Ⅱ基因型相对Ⅰ/Ⅰ基因型和Ⅰ/Ⅳ基因型的比值比是23.036倍(P<0.05),并且吸烟的比值比是15.385倍(P<0.05)。结论:IL-1RN基因多态性与早期边缘骨吸收发生有关,吸烟可能是早期边缘骨吸收发生的重要危险因素。

Objective：To investigate the relationship between the genetic polymorphism of IL-1RN gene and the marginal bone loss around the implants before stage II.Methods：A case-control study was used.Patients with marginal bone loss around implants at stage-II surgery were designated as the ＂marginal bone loss（＋）＂ group and those without bone loss as the ＂marginal bone loss（-）＂ group.Genomic DNA from buccal mucosa was amplified by the polymerase chain reaction（PCR）to detect IL-1RN Gene.Results：The patients with IL-1RN I/II genotype had a significantly higher rate of occurrence of marginal bone loss than those with IL-1RN I/I or I/IV genotype.According to the multiple logistic regression,the OR of the I/II versus the I/I＋I/IV of the IL-1RN genotype was 23.036 between the two groups and the statistical difference was significant（P0.05）.The OR of smoking was 15.385（P0.05）.Conclusion：The IL-1RN I/II genotype may be associated with early marginal bone loss.